Recombinant HSP27 Antibody HSPB1/7038R V9625 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V9625-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9625-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9625SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

Recombinant HSP27 Antibody HSPB1/7038R Detection Across Multiple Species WB. Western blot analysis of heart, skeletal muscle and skin tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit using Recombinant HSP27 Antibody clone HSPB1/7038R shows discrete bands at approximately 27-28 kDa across the tested species and tissues, consistent with the expected migration of HSPB1/HSP27. The broad detection pattern demonstrates recognition of HSPB1 in human and multiple animal species.

Recombinant HSP27 Antibody HSPB1/7038R Detection in Human Tissues and Cancer Cell Lines WB. Western blot analysis of human heart, liver, skeletal muscle and skin tissue lysates, and A549, HeLa and MCF7 cancer cell lysates using Recombinant HSP27 Antibody clone HSPB1/7038R shows a strong, discrete band at approximately 27-28 kDa in all seven samples, consistent with the expected migration of HSPB1/HSP27. Minimal background is observed across the tested human tissues and cell lines.

Recombinant HSP27 Antibody HSPB1/7038R Detection in Human Liver and MCF7 Breast Cancer Cells WB. Western blot testing of human liver tissue and MCF7 breast cancer cell lysates with Recombinant HSP27 Antibody clone HSPB1/7038R shows strong, discrete bands at approximately 25-27 kDa in both samples, consistent with the predicted molecular weight of HSPB1/HSP27. Minimal background is observed in both the tissue and cell lysates.

Recombinant HSP27 Antibody HSPB1/7038R Staining of Human Renal Cell Carcinoma IHC. Immunohistochemistry testing of FFPE human renal cell carcinoma tissue with Recombinant HSP27 Antibody clone HSPB1/7038R shows strong brown immunoreactivity in tumor cells, with a predominantly cytoplasmic staining pattern. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/7038R) as confirmation of integrity and purity.

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Recombinant HSP27 Antibody HSPB1/7038R Detection Across Multiple Species WB. Western blot analysis of heart, skeletal muscle and skin tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit using Recombinant HSP27 Antibody clone HSPB1/7038R shows discrete bands at approximately 27-28 kDa across the tested species and tissues, consistent with the expected migration of HSPB1/HSP27. The broad detection pattern demonstrates recognition of HSPB1 in human and multiple animal species.

Recombinant HSP27 Antibody HSPB1/7038R Detection in Human Tissues and Cancer Cell Lines WB. Western blot analysis of human heart, liver, skeletal muscle and skin tissue lysates, and A549, HeLa and MCF7 cancer cell lysates using Recombinant HSP27 Antibody clone HSPB1/7038R shows a strong, discrete band at approximately 27-28 kDa in all seven samples, consistent with the expected migration of HSPB1/HSP27. Minimal background is observed across the tested human tissues and cell lines.

Recombinant HSP27 Antibody HSPB1/7038R Detection in Human Liver and MCF7 Breast Cancer Cells WB. Western blot testing of human liver tissue and MCF7 breast cancer cell lysates with Recombinant HSP27 Antibody clone HSPB1/7038R shows strong, discrete bands at approximately 25-27 kDa in both samples, consistent with the predicted molecular weight of HSPB1/HSP27. Minimal background is observed in both the tissue and cell lysates.

Recombinant HSP27 Antibody HSPB1/7038R Staining of Human Renal Cell Carcinoma IHC. Immunohistochemistry testing of FFPE human renal cell carcinoma tissue with Recombinant HSP27 Antibody clone HSPB1/7038R shows strong brown immunoreactivity in tumor cells, with a predominantly cytoplasmic staining pattern. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/7038R) as confirmation of integrity and purity.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat, Rabbit, Hamster, Guinea pig
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	HSPB1/7038R
Purity	Protein A/G affinity
UniProt	P04792
Localization	Cytoplasm, Nucleus
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml (Human) Western Blot : 2-4ug/ml (Human/Mouse/Rat/Rabbit/Hamster/Guinea pig
Limitations	This Recombinant HSP27 Antibody HSPB1/7038R / Recombinant HSPB1 Antibody is available for research use only.
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Description

Recombinant HSP27 Antibody HSPB1/7038R / Recombinant HSPB1 Antibody recognizes HSPB1, a small heat shock protein widely known as HSP27. HSPB1 functions as an ATP-independent molecular chaperone and contributes to cellular mechanisms responsible for maintaining protein homeostasis. Its activity is particularly important during conditions that challenge protein stability, when HSP27 can interact with destabilized proteins and help limit inappropriate aggregation.

HSPB1 contains a conserved alpha-crystallin domain characteristic of the small heat shock protein family. Unlike ATP-dependent chaperones, HSP27 can associate with partially unfolded client proteins without directly driving an ATP-dependent refolding reaction. These interactions can stabilize vulnerable proteins and facilitate their subsequent processing by other components of the protein quality-control machinery. HSPB1 also assembles into dynamic oligomeric complexes whose organization and function can be regulated by phosphorylation.

HSP27 participates in cellular responses to heat shock, oxidative stress and other environmental or physiological challenges. In addition to its molecular chaperone activity, HSPB1 has been studied for roles in cytoskeletal organization, cell migration, apoptosis and survival. Changes in phosphorylation and oligomerization allow HSP27 to alter its interactions in response to stress-responsive signaling, connecting protein quality control with broader mechanisms of cellular adaptation.

HSPB1 is also widely investigated in cancer biology. Altered HSP27 expression and activity have been reported in multiple malignancies and studied in relation to tumor cell survival, migration, resistance to apoptosis and therapeutic response. HSPB1 is additionally relevant to neurological research because pathogenic variants can cause inherited peripheral neuropathies, including forms of Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

NSJ Bioreagents offers this recombinant rabbit monoclonal Recombinant HSP27 Antibody as clone HSPB1/7038R. Recombinant monoclonal production provides a defined antibody sequence and supports consistent reagent manufacture while retaining monoclonal target recognition. A Recombinant HSP27 Antibody provides a research tool for investigating HSPB1 expression, small heat shock protein biology, molecular chaperone activity, proteostasis and cellular stress responses.

For an additional monoclonal antibody to HSPB1 with Protein Microarray Validated specificity data, see our HSPB1 Antibody / Heat Shock Protein Beta-1 Antibody page.

Application Notes

Optimal dilution of the Recombinant HSP27 Antibody HSPB1/7038R / Recombinant HSPB1 Antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the Recombinant HSP27 Antibody HSPB1/7038R.

Storage

Aliquot the Recombinant HSP27 Antibody HSPB1/7038R and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

HSPB1 antibody, HSP27 antibody, Heat shock protein beta-1 antibody, Heat shock 27 kDa protein antibody, HSP28 antibody, HSP25 antibody, Stress-responsive protein 27 antibody

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Recombinant HSP27 Antibody HSPB1/7038R / Recombinant HSPB1 Antibody [clone HSPB1/7038R] (V9625)

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Immunogen

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