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Home >> Antibodies >> Recombinant HER2 Antibody / ErbB2 / Extracellular region

Recombinant HER2 Antibody / ErbB2 / Extracellular region [clone ERBB2/13055R] (V5689)

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  Catalog No Formulation Size Price (USD)  
Image V5689-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5689-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5689SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human breast cancer tissue with recombinant HER-2 antibody (clone ERBB2/13055R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with recombinant HER-2 antibody (clone ERBB2/13055R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HER-2 antibody (clone ERBB2/13055R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ERBB2/13055R
UniProt P04626
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant HER-2 antibody is available for research use only.
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Description

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

Optimal dilution of the recombinant HER-2 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein from human ERBB2 corresponding to the extracellular domain (ECD) was used as the immunogen for the recombinant HER-2 antibody.

Storage

Aliquot the recombinant HER-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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