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Home >> Antibodies >> Recombinant GATA3 Antibody / GATA-binding factor 3

Recombinant GATA3 Antibody / GATA-binding factor 3 [clone rGATA3/9107] (V5519)

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  Catalog No Formulation Size Price (USD)  
Image V5519-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5519-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5519SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
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GATA3 Antibody Human Kidney and HeLa WB. Western blot analysis of human kidney and HeLa cell lysates using recombinant GATA3 Antibody / GATA Binding Protein 3 Antibody clone rGATA3/9107. A distinct band is detected at approximately 40 kDa, consistent with reported migration patterns of GATA binding protein 3 / GATA3, a zinc finger transcription factor involved in epithelial differentiation and T-cell lineage regulation.
SDS-PAGE analysis of purified, BSA-free GATA3 antibody (clone rGATA3/9107) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rGATA3/9107
Purity Protein A/G affinity
UniProt P23771
Localization Nucleus
Applications Western Blot : 2-4ug/ml
Limitations This recombinant GATA3 antibody is available for research use only.
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Description

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

For highly selective detection of GATA3 in breast lineage-associated and epithelial differentiation studies, see the GATA3 Antibody / Breast Lineage Marker Antibody clone GATA3/2444 featuring protein microarray specificity validation.

Application Notes

Optimal dilution of the recombinant GATA3 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 200-300) of human GATA-3 protein was used as the immunogen for the recombinant GATA3 antibody.

Storage

Aliquot the recombinant GATA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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