- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Estrogen Receptor alpha Antibody N-Terminal Region [clone RM292] (R20308)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20308-0.1ML | Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ul | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM292 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P03372 |
| Localization | Nuclear |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 Western Blot : 1:200-1:500 |
| Limitations | This Estrogen Receptor alpha antibody is available for research use only. |
|
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Estrogen Receptor alpha Antibody N-Terminal Region recombinant mAb recognizes Estrogen Receptor alpha, a ligand-activated nuclear transcription factor encoded by the ESR1 gene on chromosome 6q25.1. Estrogen Receptor alpha, also referred to as ER alpha or ESR1, belongs to the nuclear receptor superfamily and regulates gene expression in response to estrogen signaling. This recombinant monoclonal antibody is directed against the N-terminal region of ER alpha, a domain that contains activation function 1 and contributes to transcriptional regulation independently of ligand binding.
The N-terminal domain of Estrogen Receptor alpha plays a critical role in modulating receptor activity through interactions with coactivator proteins and components of the transcriptional machinery. Unlike the ligand-binding domain located in the C-terminal portion of the receptor, the N-terminal activation function 1 domain contributes to transcriptional activity in a context-dependent manner and can influence cell type-specific gene expression patterns. Targeting the N-terminal region allows investigation of receptor isoforms, structural integrity, and potential truncation variants that may alter functional signaling dynamics.
Estrogen Receptor alpha contains multiple functional domains, including a DNA-binding domain with zinc finger motifs, a hinge region involved in nuclear localization, and a ligand-binding domain that mediates estrogen interaction. Upon estrogen binding, ESR1 undergoes conformational changes that promote receptor dimerization and binding to estrogen response elements within promoter regions of target genes. These interactions regulate transcriptional programs controlling proliferation, differentiation, and survival in hormone-responsive tissues.
Expression of ER alpha is most prominent in breast epithelium, endometrium, ovary, and additional reproductive tissues. In oncology research, ESR1 expression status is a central biomarker in breast carcinoma and other estrogen-dependent malignancies. Detection of the N-terminal region of ER alpha can support studies focused on receptor structure, isoform characterization, and estrogen-driven transcriptional regulation. This Estrogen Receptor alpha Antibody N-Terminal Region recombinant mAb targets ESR1 protein in research applications involving cellular and tissue-based analysis of hormone signaling pathways.
The stated application concentrations are suggested starting points. Titration of the recombinant Estrogen Receptor alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A peptide corresponding to residues near the N-terminus of human ER-alpha was used as the immunogen for the Estrogen Receptor alpha antibody.
Store the Estrogen Receptor alpha antibody at -20oC.
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