Recombinant E-Cadherin Antibody [clone rCDH1/1525] (V3564)

Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (green, clone rCDH1/1525) and Reddot nuclear stain (red).

IHC testing of formalin-fixed, paraffin-embedded human colon carcinoma with recombinant E-Cadherin antibody (clone rCDH1/1525). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone rCDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant E-Cadherin antibody (clone rCDH1/1525). These results demonstrate the foremost specificity of the rCDH1/1525 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone rCDH1/1525) as confirmation of integrity and purity.