Cytokeratin 16 Antibody rKRT16/1714 / CK16 Monoclonal Antibody [clone rKRT16/1714] (V7297)

Cytokeratin 16 Antibody rKRT16/1714 by IHC in human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil tissue using Cytokeratin 16 Antibody clone rKRT16/1714. Brown cytoplasmic immunoreactivity is concentrated within epithelial structures, highlighting cells associated with the stratified squamous epithelial compartment. The staining follows portions of the epithelial architecture, while surrounding lymphoid tissue exhibits comparatively little immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue, providing contrast with the positively stained epithelial cells. The CK16 Monoclonal Antibody was applied following heat-induced epitope retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 16 Antibody clone rKRT16/1714 under non-reduced (NR) and reduced (R) conditions. The non-reduced sample shows a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reduced conditions, distinct bands migrate at approximately 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains. Molecular weight standards are included for reference. This analysis evaluates the electrophoretic characteristics and purity of the CK16 Monoclonal Antibody preparation rather than detection of endogenous cytokeratin 16 protein.