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Home >> Antibodies >> CK20 Antibody / Cytokeratin 20 Antibody

CK20 Antibody / Cytokeratin 20 Antibody [clone KRT20/3129R] (V8313)

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  Catalog No Formulation Size Price (USD)  
Image V8313-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8313-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8313SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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CK20 Antibody / Cytokeratin 20 (clone KRT20/3129R). Immunohistochemistry analysis of FFPE human colon tissue using recombinant rabbit monoclonal CK20 antibody (clone KRT20/3129R). HRP-DAB brown chromogenic staining highlights cytoplasmic Keratin 20 / Cytokeratin 20 (KRT20) expression in epithelial cells lining colonic glandular crypt structures, while surrounding stromal cells remain largely negative. The staining pattern reflects the expected epithelial localization of this intermediate filament protein in gastrointestinal mucosa. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant CK20 antibody (clone KRT20/3129R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
CK20 Antibody / Cytokeratin 20 (clone KRT20/3129R). Western blot analysis of human HT-29 cell lysate using CK20 antibody (clone KRT20/3129R). Lane 1: human HT-29 cell lysate. A band is detected at approximately 46 kDa, consistent with the predicted molecular weight of Keratin 20 / Cytokeratin 20 (KRT20), an epithelial intermediate filament protein commonly expressed in gastrointestinal epithelial cells such as HT-29 colorectal adenocarcinoma cells.
SDS-PAGE analysis of purified, BSA-free recombinant CK20 antibody (clone KRT20/3129R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name KRT20/3129R
Purity Protein A affinity chromatography
UniProt P35900
Localization Cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CK20 antibody is available for research use only.
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Description

Keratin 20 (KRT20) is a type I acidic cytokeratin that belongs to the epithelial intermediate filament protein family responsible for maintaining cytoskeletal stability and structural organization in epithelial cells. CK20 Antibody / Cytokeratin 20 (clone KRT20/3129R) recognizes Keratin 20 and enables detection of this epithelial cytoskeletal protein in studies examining epithelial differentiation, epithelial cell identity, and cytoskeletal organization. Keratin 20 is primarily localized within the cytoplasm of epithelial cells where it forms intermediate filament networks that provide mechanical support and help maintain epithelial tissue architecture.

Keratin 20 is encoded by the KRT20 gene located on chromosome 17q21 within a genomic cluster containing several keratin genes involved in epithelial cytoskeletal organization. The protein is widely referred to in the literature as Cytokeratin 20 or CK20, both common synonyms frequently used in epithelial biology and tumor marker research. Keratin 20 forms heterodimers with type II keratins such as keratin 8, and these dimers polymerize to generate intermediate filament networks throughout the cytoplasm of epithelial cells. These filament structures support epithelial cell stability and contribute to the mechanical resilience of tissues that experience continuous mechanical stress.

Keratin 20 expression is strongly associated with differentiated epithelial cell populations. High expression levels are observed in intestinal epithelial cells, gastric mucosa, and urothelial umbrella cells. Because of this restricted distribution pattern, Cytokeratin 20 antibody reagents are frequently used as markers for epithelial lineage identification. Detection of KRT20 protein therefore supports studies examining epithelial differentiation, epithelial organization, and epithelial tissue development.

In cancer biology, Keratin 20 expression is frequently retained in tumors originating from gastrointestinal and urothelial epithelia. CK20 expression is commonly detected in colorectal carcinoma, gastric carcinoma, pancreatic carcinoma, and bladder carcinoma, where it serves as a marker of epithelial differentiation. Detection of Cytokeratin 20 assists research examining epithelial tumor biology, cytoskeletal organization, and differentiation states within tumor cells.

The recombinant rabbit monoclonal antibody clone KRT20/3129R targets Keratin 20 and can be used to detect KRT20 protein in research applications examining epithelial cytoskeletal proteins. Detection of Cytokeratin 20 supports studies investigating epithelial differentiation pathways, epithelial tissue organization, and epithelial tumor biology.

Application Notes

Optimal dilution of the CK20 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.

Storage

Store the recombinant CK20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 20 antibody, KRT20 antibody, Cytokeratin-20 antibody, CK20 protein antibody, Keratin 20 protein antibody

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