CDH2 Antibody / Cadherin-2 / N-Cadherin [clone rCDH2/8291] (V4565)

CDH2 Antibody Tissue WB. Western blot analysis of N-Cadherin / CDH2 expression across multi-species heart tissue lysates using recombinant mouse monoclonal CDH2 antibody clone rCDH2/8291. Lane 1: human heart lysate, Lane 2: mouse heart lysate, Lane 3: rat heart lysate, Lane 4: hamster heart lysate, Lane 5: guinea pig heart lysate, Lane 6: rabbit heart lysate. A band is detected at approximately 120-135 kDa, consistent with the predicted molecular weight of N-Cadherin (CDH2), with the slightly higher apparent size reflecting known glycosylation of this transmembrane adhesion protein. The consistent band pattern across species aligns with the highly conserved role of N-Cadherin in cardiac muscle cell-cell adhesion and intercalated disc structure.

CDH2 Antibody Tissue WB. Western blot analysis of N-Cadherin / CDH2 expression in human heart tissue lysate using recombinant mouse monoclonal CDH2 antibody clone rCDH2/8291. Lane 1: human heart lysate. A band is detected at approximately 120-135 kDa, consistent with the predicted molecular weight of N-Cadherin (CDH2), with the slightly higher apparent migration reflecting known glycosylation of this membrane-associated adhesion protein. The strong signal in heart tissue is expected given the role of N-Cadherin in maintaining cardiomyocyte cell-cell junctions within intercalated discs.

CDH2 Antibody Kidney Tissue IHC. Immunohistochemical analysis of N-Cadherin / CDH2 expression in formalin-fixed, paraffin-embedded human kidney tissue using recombinant CDH2 antibody clone rCDH2/8291. Distinct membranous and cytoplasmic staining is observed in renal tubular epithelial cells, highlighting cell-cell junctions consistent with the known localization of N-Cadherin in epithelial adhesion structures. Glomerular regions show minimal to low staining. The inset represents a negative control with primary antibody omitted, demonstrating low background signal. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CDH2 antibody (clone rCDH2/8291) as confirmation of integrity and purity.