Recombinant CD31 Antibody C31/1395R / PECAM-1 Recombinant Antibody [clone C31/1395R] (V7243)

Description

Recombinant CD31 Antibody C31/1395R / PECAM-1 Recombinant Antibody recognizes platelet endothelial cell adhesion molecule 1, a transmembrane glycoprotein encoded by the PECAM1 gene. Commonly known as CD31, PECAM-1 is a member of the immunoglobulin superfamily and is strongly associated with vascular endothelial cells. It is also expressed by platelets and several leukocyte populations, making the protein relevant to vascular biology, hematopoiesis, inflammation and immune cell trafficking.

PECAM-1 contains six extracellular immunoglobulin-like domains, a single transmembrane region and a cytoplasmic domain containing regulatory tyrosine residues. Homophilic interactions between CD31 molecules contribute to adhesion between neighboring endothelial cells, particularly at intercellular junctions. Its intracellular region participates in phosphorylation-dependent signaling and can influence cellular responses to mechanical forces, inflammatory signals and changes in cell adhesion.

CD31 contributes to the maintenance of endothelial barrier integrity and the movement of leukocytes across vascular endothelium. During inflammatory responses, circulating immune cells migrate through endothelial junctions in a coordinated process involving multiple adhesion molecules. PECAM-1 participates in this transendothelial migration and helps regulate interactions between endothelial and hematopoietic cells. A PECAM-1 Recombinant Antibody can therefore support investigation of vascular inflammation, endothelial junction biology and leukocyte trafficking.

CD31 is also widely studied as a marker of vascular endothelial cells. Analysis of its expression is commonly used to investigate angiogenesis, vascular development and changes in blood vessel organization. PECAM-1 is consequently relevant to studies of tumor vascularization, tissue remodeling and endothelial differentiation. Its additional expression among platelets and selected leukocyte populations extends its biological significance beyond the vascular endothelium.

NSJ Bioreagents offers recombinant clone C31/1395R for research into CD31 expression and PECAM-1 biology. The Recombinant CD31 Antibody designation emphasizes both the widely recognized CD name and recombinant format, while PECAM-1 Recombinant Antibody incorporates the alternative protein terminology.

A Recombinant CD31 Antibody provides a recombinant reagent for investigating endothelial cells, angiogenesis and vascular organization, while a PECAM-1 Recombinant Antibody supports research into cell adhesion, vascular integrity and leukocyte trafficking.

For a Protein Microarray Validated monoclonal antibody with CD31 as the primary designation, see our CD31 Antibody PECAM1/3527 / PECAM-1 Monoclonal Antibody.

Application Notes

Titering of the Recombinant CD31 Antibody C31/1395R / PECAM-1 Recombinant Antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human full length recombinant protein was used as the immunogen for the Recombinant CD31 Antibody C31/1395R.

Storage

Store the Recombinant CD31 Antibody C31/1395R at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PECAM-1 antibody, PECAM1 antibody, Platelet Endothelial Cell Adhesion Molecule antibody, EndoCAM antibody, GPIIA' antibody