- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Beta Actin Antibody (Loading Control) [clone RM112] (R20197)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20197-0.1ML | Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ul | 429 |
| Availability | 1-3 business days |
| Species Reactivity | All Species |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM112 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P60709 |
| Gene ID | 60 |
| Applications | Western Blot : 1:1000 Immunoprecipitation : 1:200 Chromatin IP (ChIP) : 1:200 Immunocytochemistry : 1:200 Immunohistochemistry : 1:200-1:1000 |
| Limitations | This recombinant Beta Actin antibody is available for research use only. |
|
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The Recombinant Beta Actin antibody is a recombinant reagent that specifically recognizes beta actin, one of the most abundant and highly conserved cytoskeletal proteins in eukaryotic cells. Beta actin is encoded by the ACTB gene and plays a central role in maintaining cell shape, enabling motility, and supporting intracellular transport. It polymerizes into microfilaments that form part of the actin cytoskeleton, a dynamic network that coordinates with microtubules and intermediate filaments to regulate essential cellular processes. Because of its stable and constitutive expression in nearly all cell types, beta actin is widely used as a loading control in western blotting, making the Recombinant Beta Actin antibody a critical tool in molecular biology and cell biology research.
Structurally, actin exists in two forms: monomeric globular actin (G-actin) and filamentous actin (F-actin). The polymerization of G-actin into F-actin is tightly regulated by actin-binding proteins and signaling pathways, allowing rapid reorganization of the cytoskeleton during cell migration, endocytosis, or cytokinesis. Beta actin differs slightly from other actin isoforms, such as gamma actin, in amino acid composition and tissue distribution, yet its conservation across species underscores its fundamental role in cell biology. The Recombinant Beta Actin antibody targets epitopes within this protein to ensure reliable detection in multiple applications.
The Recombinant Beta Actin antibody is used extensively in western blotting, where it serves as a standard loading control for protein quantification. Its stable expression across most cell types allows normalization of protein levels, ensuring that differences in signal intensity reflect experimental variables rather than unequal sample loading. In immunofluorescence, the antibody labels the filamentous actin cytoskeleton, enabling visualization of structural organization and cytoskeletal remodeling. In immunohistochemistry, the Recombinant Beta Actin antibody highlights actin-rich regions in tissues, providing insight into developmental processes and pathological changes. Recombinant production ensures consistency between lots, eliminating the variability that may occur with hybridoma-derived antibodies.
This antibody is particularly valuable in studies of cancer, neurobiology, and infectious disease, where actin dynamics are frequently altered. It also plays an important role in drug discovery, as many therapeutic agents influence cytoskeletal stability and function. By serving as a dependable loading control, the Recombinant Beta Actin antibody improves the accuracy and reproducibility of quantitative analyses. Synonym phrases such as recombinant ACTB antibody and recombinant beta cytoskeletal actin antibody broaden product visibility for researchers searching under alternate terminology.
By providing validated and reproducible detection, the Recombinant Beta Actin antibody supports high-quality data generation across diverse experimental platforms. NSJ Bioreagents ensures strict quality control, giving scientists confidence in its application as both a cytoskeletal marker and a reliable loading control. With this reagent, researchers can study actin biology while ensuring accuracy in protein quantification assays.
This recombinant Beta Actin antibody reacts to b-Actin (ACTB/Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
The stated application concentrations are suggested starting points. Titration of the recombinant Beta Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A peptide corresponding to the N-terminus of b-Actin was used as the immunogen for this recombinant Beta Actin antibody.
Store the recombinant Beta Actin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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