Recombinant Beta-2 Microglobulin Antibody

	Catalog No	Formulation	Size	Price (USD)
	V3538-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	539
Formulation
	V3538-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	249
Formulation
	V3538SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	539
Formulation
	V3538IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	539
Formulation

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Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Beta-2 Microglobulin antibody (clone rB2M/961); Red=isotype control, Blue= recombinant Beta-2 Microglobulin antibody.

IHC testing of FFPE human lung carcinoma stained with recombinant Beta-2-Microglobulin antibody (rB2M/961). Required HEIR: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma stained with recombinant Beta-2-Microglobulin antibody (rB2M/961). Required HEIR: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human kidney lysate with recombinant Beta-2 Microglobulin antibody (clone rB2M/961). Expected molecular weight: 12-14 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Beta-2 Microglobulin antibody (clone rB2M/961). These results demonstrate the foremost specificity of the rB2M/961 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Beta-2 Microglobulin antibody (clone rB2M/961) as confirmation of integrity and purity.

IHC testing of FFPE human cervical carcinoma stained with recombinant Beta-2-Microglobulin antibody (rB2M/961). Required HEIR: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human HeLa cells with recombinant Beta-2 Microglobulin antibody (clone rB2M/961).

Western blot testing of human THP-1 and Raji cell lysate with recombinant Beta-2 Microglobulin antibody. Expected molecular weight: 12-14 kDa.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Clonality	Recombinant Mouse Monoclonal
Isotype	Mouse IgG2b, kappa
Clone Name	rB2M/961
Purity	Protein G affinity chromatography
UniProt	P61769
Localization	Cytoplasmic
Applications	Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 1-2ug/ml
Limitations	This recombinant Beta-2 Microglobulin antibody is available for research use only.
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Description

Recombinant Beta-2 Microglobulin antibody is a reagent designed to detect beta-2 microglobulin, a small non glycosylated protein that forms the light chain of MHC class I molecules. Encoded by the B2M gene, beta-2 microglobulin associates with the heavy chain of MHC class I to stabilize its structure and present peptide antigens to cytotoxic T cells. It is expressed on nearly all nucleated cells, making it a universal marker for MHC class I expression.

Beta-2 microglobulin plays critical roles in antigen presentation and immune surveillance. By stabilizing MHC class I complexes, it ensures efficient recognition of infected or transformed cells by CD8 positive T lymphocytes. Beyond immunity, beta-2 microglobulin circulates in serum and is elevated in conditions such as renal dysfunction, inflammation, and malignancy. It is also linked to amyloidosis when abnormal protein deposition occurs.

The Recombinant Beta-2 Microglobulin antibody clone rB2M/961 provides high specificity and consistency. Recombinant production minimizes variability between lots and supports reproducible research outcomes. Clone rB2M/961 has been applied in studies of antigen presentation, lymphocyte biology, and disease monitoring. Its utility extends from basic immunology to clinical investigations, where beta-2 microglobulin serves as a biomarker of disease activity.

Elevated serum levels of beta-2 microglobulin are observed in kidney disease, multiple myeloma, and certain lymphomas. Research using clone rB2M/961 has clarified how alterations in beta-2 microglobulin expression or turnover contribute to pathology. In transplantation biology, beta-2 microglobulin detection provides information about immune compatibility and graft outcomes. Its broad relevance underscores the importance of this antibody in multiple areas of research.

NSJ Bioreagents supplies this Recombinant Beta-2 Microglobulin antibody to support investigations into antigen presentation, immune surveillance, and disease mechanisms. The protein is also referred to as B2M antibody, MHC class I light chain antibody, beta chain of histocompatibility antigen antibody, and amyloid associated protein antibody, highlighting the varied contexts in which it is studied.

Application Notes

Optimal dilution of the recombinant Beta-2 Microglobulin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.