BCL2L4 Antibody / BAX [clone BAX/9925R] (V5784)

BCL2L4 Antibody Human Liver IHC. Immunohistochemical staining of FFPE human liver tissue with recombinant BCL2L4 antibody, clone BAX/9925R. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes prior to staining. Strong cytoplasmic immunoreactivity is observed in hepatocytes, consistent with the expected localization of BAX, a pro-apoptotic BCL2 family protein that regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway. Cell nuclei are counterstained with hematoxylin (blue). Inset: PBS was used in place of the primary antibody as a negative control.

BCL2L4 Antibody Human A549 and MCF7 WB. Western blot testing of 1) human A549 and 2) human MCF7 cell lysates with recombinant BCL2L4 antibody, clone BAX/9925R. A distinct immunoreactive band is detected at approximately 21 kDa in both cell lines, consistent with the predicted molecular weight of BAX. The results demonstrate specific detection of this pro-apoptotic BCL2 family protein, which regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway in response to cellular stress.

BCL2L4 Antibody Human A549 Cell WB. Western blot testing of human A549 cell lysate with recombinant BCL2L4 antibody, clone BAX/9925R. A distinct immunoreactive band is detected at approximately 21 kDa, consistent with the predicted molecular weight of BAX. The results demonstrate specific detection of this pro-apoptotic BCL2 family protein, which regulates mitochondrial outer membrane permeabilization and activation of the intrinsic apoptotic pathway in response to cellular stress.

SDS-PAGE analysis of purified, BSA-free recombinant BCL2L4 antibody (clone BAX/9925R) as confirmation of integrity and purity.