Beta-Catenin Antibody / CTNNB1 Antibody [clone CTNNB1/2030R] (V3600)

Beta-Catenin Antibody Cervical Carcinoma IHC. Immunohistochemistry of human cervical carcinoma using Beta-Catenin Antibody (clone CTNNB1/2030R) demonstrates strong membranous and cytoplasmic staining in tumor epithelial cells, consistent with CTNNB1 / Catenin beta-1 expression in adherens junctions, with focal areas suggesting increased cytoplasmic accumulation. Nuclear counterstain highlights tissue architecture, and stromal regions show comparatively minimal signal. This staining pattern reflects the dual role of beta-catenin in cell adhesion and Wnt signaling-associated redistribution. HIER: boil FFPE tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Beta-Catenin Antibody Tonsil IHC. Immunohistochemistry of human tonsil tissue using Beta-Catenin Antibody (clone CTNNB1/2030R) shows distinct membranous staining outlining epithelial cell borders within crypt regions, consistent with CTNNB1 / Catenin beta-1 localization at adherens junctions, with mild cytoplasmic signal in select epithelial cells. Lymphoid compartments display minimal staining, highlighting tissue-specific expression patterns. Hematoxylin counterstain provides nuclear contrast for structural context. HIER: boil FFPE tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Beta-Catenin Antibody HeLa Cell IF. Immunofluorescence analysis of PFA-fixed human HeLa cells using Beta-Catenin Antibody (clone CTNNB1/2030R) shows prominent membranous and cytoplasmic green fluorescence consistent with CTNNB1 / Catenin beta-1 localization at cell-cell junctions, outlining cell borders with additional intracellular signal. Reddot nuclear stain (red) clearly marks nuclei, providing contrast to the peripheral beta-catenin distribution. The observed pattern reflects the role of beta-catenin in adherens junction organization and cytoplasmic signaling pools.

Beta-Catenin Antibody HeLa Cell FACS. Flow cytometry analysis of PFA-fixed human HeLa cells using Beta-Catenin Antibody (clone CTNNB1/2030R) shows a clear rightward shift of the blue population relative to the red isotype control, indicating specific detection of CTNNB1 / Catenin beta-1 expression. The signal distribution supports intracellular localization consistent with beta-catenin’s cytoplasmic and membrane-associated pools following fixation and permeabilization. Red represents isotype control and blue represents recombinant beta-catenin antibody staining.

Beta-Catenin Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using Beta-Catenin Antibody (clone CTNNB1/2030R) detects a prominent band at approximately 90-95 kDa, consistent with the predicted molecular weight of Catenin beta-1 / CTNNB1 at ~85 kDa with a modest upward shift commonly observed in SDS-PAGE. This migration difference is consistent with known phosphorylation of beta-catenin, which can alter electrophoretic mobility. The single dominant band supports specific detection of CTNNB1 in HeLa cells.

Beta-Catenin Antibody HuProt Microarray Specificity. Protein microarray analysis using Beta-Catenin Antibody (clone CTNNB1/2030R) demonstrates highly specific binding to CTNNB1 / Catenin beta-1, with the target protein ranked as the top hit and showing a strong Z score relative to all other proteins on the array. Signal intensity drops sharply for non-target proteins, supporting selective recognition with minimal off-target interaction. Z score represents the strength of signal in standard deviations above the mean of all array signals, while S score reflects the separation between the top-ranked target and the next highest signal, indicating relative specificity.

SDS-PAGE analysis of purified, BSA-free recombinant b-Catenin antibody (clone CTNNB1/2030R) as confirmation of integrity and purity.

Beta-Catenin Antibody Brain WB. Western blot analysis of human brain tissue lysate using Beta-Catenin Antibody (clone CTNNB1/2030R) detects a clear band at approximately 90-95 kDa, consistent with the predicted molecular weight of Catenin beta-1 / CTNNB1 at ~85 kDa with a commonly observed upward shift during SDS-PAGE. This shift is consistent with known phosphorylation of beta-catenin, which can affect electrophoretic mobility. The band pattern aligns with expected expression of CTNNB1 in neural tissue.