Recombinant b-Catenin Antibody [clone CTNNB1/2030R] (V3600)

IHC testing of FFPE human cervical carcinoma with recombinant b-Catenin antibody (clone CTNNB1/2030R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human tonsil tissue with recombinant b-Catenin antibody (clone CTNNB1/2030R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant b-Catenin antibody (clone CTNNB1/2030R, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant b-Catenin antibody (clone CTNNB1/2030R); Red=isotype control, Blue= recombinant b-Catenin antibody.

Western blot testing of human HeLa cell lysate with recombinant b-Catenin antibody (clone CTNNB1/2030R). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant b-Catenin antibody (clone CTNNB1/2030R). These results demonstrate the foremost specificity of the CTNNB1/2030R mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant b-Catenin antibody (clone CTNNB1/2030R) as confirmation of integrity and purity.

Western blot testing of human brain tissue lysate with recombinant b-Catenin antibody (clone CTNNB1/2030R). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.