• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant AKT1 Antibody

Recombinant AKT1 Antibody [clone rAKT1/2491] (V7745)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V7745-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
Image
V7745-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
Image
V7745SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
Bulk quote request
›
IHC staining of FFPE human prostate carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
AKT1 Antibody Colon Carcinoma IHC. Immunohistochemistry staining of FFPE human colon carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone rAKT1/2491) as confirmation of integrity and purity.
Western blot testing of human MCF7 cell lysate with AKT1 antibody. Predicted molecular weight ~56 kDa.
AKT1 Antibody Human Tissue WB. Western blot analysis of human brain, kidney, liver, lung, pancreas, skeletal muscle, and spleen tissue lysates using AKT1 antibody (clone rAKT1/2491) detects a band at approximately 55-60 kDa, consistent with the predicted molecular weight of AKT1, with broad expression observed across tissues.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rAKT1/2491
Purity Protein G affinity chromatography
UniProt P31749
Localization Nucleus, plasma membrane, cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 1-2ug/ml
Limitations This recombinant AKT1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, ELISA
    Reactivity : Human
    Recrabbitmono
  • Applications : WB, IHC-P, IF
    Reactivity : Human, Mouse, Rat
    Recrabbitmono
  • Applications : IHC-P, WB
    Reactivity : Human
    Microvalidated
  • Applications : WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse
    Microvalidated
  • Applications : WB
    Reactivity : Human, Mouse, Rat, Chicken
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine
  • Applications : IHC-P
    Reactivity : Human
  • Applications : WB, DB, ELISA
    Reactivity : Human
  • Applications : IHC, FACS, WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine, Xenopus
  • Applications : IF, FACS, WB, IHC, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat, Bovine
  • Applications : WB, IHC, ELISA
    Reactivity : Mouse
    Pred. Reactivity : Human
  • Applications : WB, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat
  • Applications : IHC, WB, IF, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Bovine
  • Applications : WB, IF, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat

Description

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

For a microarray-validated AKT1 antibody supporting high-specificity detection, see our AKT1 antibody (clone AKT1/2552).

Application Notes

Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.

Immunogen

Amino acids 85-189 from the human protein were used as the immunogen for the recombinant AKT1 antibody.

Storage

Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all AKT1 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.