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Home >> Antibodies >> Recombinant AKT1 Antibody

Recombinant AKT1 Antibody [clone AKT1/3898R] (V8442)

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  Catalog No Formulation Size Price (USD)  
Image V8442-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8442-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8442SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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AKT1 Antibody Multi-Tissue WB. Western blot analysis of human brain, kidney, liver, lung, pancreas, skeletal muscle, and spleen tissue lysates using AKT1 antibody (clone AKT1/3898R) detects a band at approximately 55-60 kDa, consistent with the predicted molecular weight of AKT1, with uniform expression observed across tissues.
SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone AKT1/3898R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AKT1/3898R
Purity Protein A affinity chromatography
UniProt P31749
Localization Nuclear, cytoplasmic, cell surface
Applications Western Blot : 2-4ug/ml
ELISA : order Ab without BSA for coating
Limitations This recombinant AKT1 antibody is available for research use only.
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Description

Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

For a microarray-validated AKT1 antibody supporting high-specificity detection, see our AKT1 antibody (clone AKT1/2552).

Application Notes

Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.

Storage

Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all AKT1 antibodies

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