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Home >> Antibodies >> PRODH Antibody

PRODH Antibody (F54259)

  Catalog No Formulation Size Price (USD)  
Image F54259-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F54259-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Western blot testing of human brain lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
Western blot testing of human MDA-MB-468 cell lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
Western blot testing of mouse 1) liver and 2) kidney lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
IHC testing of FFPE human skeletal muscle tissue with PRODH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with PRODH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human A549 cells with PRODH antibody; Blue=isotype control, Green= PRODH antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9WU79
Gene ID 19125
Localization Cytoplasmic
Applications Western Blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:25-1:100
Flow Cytometry : 1:25 (1x10e6 cells)
Limitations This PRODH antibody is available for research use only.
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Description

PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PRODH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 130-155 from the human protein were used as the immunogen for the PRODH antibody.

Storage

Aliquot the PRODH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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