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Home >> Antibodies >> PRODH Antibody

PRODH Antibody (F54259)

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  Catalog No Formulation Size Price (USD)  
Image F54259-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54259-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human brain lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
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Western blot testing of human brain lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
Western blot testing of human MDA-MB-468 cell lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
Western blot testing of mouse 1) liver and 2) kidney lysate with PRODH antibody. Predicted molecular weight: 68 kDa.
IHC testing of FFPE human skeletal muscle tissue with PRODH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with PRODH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human A549 cells with PRODH antibody; Blue=isotype control, Green= PRODH antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9WU79
Gene ID 19125
Localization Cytoplasmic
Applications Western blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:25-1:100
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This PRODH antibody is available for research use only.
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Description

PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PRODH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 130-155 from the human protein were used as the immunogen for the PRODH antibody.

Storage

Aliquot the PRODH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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