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Home >> Antibodies >> PPP2R5D Antibody

PPP2R5D Antibody (RQ8110)

  Catalog No Formulation Size Price (USD)  
Image RQ8110 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human SiHa cells with PPP2R5D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R5D antibody.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human HepG2, 4) rat brain, 5) rat PC-12, 6) mouse brain and 7) mouse Neuro-2a cell lysate with PPP2R5D antibody. Predicted molecular weight ~58 kDa, ~66 kDa and ~70 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q14738
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PPP2R5D antibody is available for research use only.
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Description

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform is an enzyme that in humans is encoded by the PPP2R5D gene. The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Notes

Optimal dilution of the PPP2R5D antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R91-L602) was used as the immunogen for the PPP2R5D antibody.

Storage

After reconstitution, the PPP2R5D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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