PPIA Antibody / Cyclophilin A (F54802)

PPIA Antibody A431 and HeLa WB. Western blot analysis of 1) human A431 and 2) human HeLa cell lysates using PPIA Antibody demonstrates strong immunoreactive bands at approximately 18 kDa in both cell lines, consistent with the expected molecular weight of PPIA (Cyclophilin A). PPIA is a ubiquitously expressed peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress response pathways. The detection of a single prominent band in both epithelial-derived cell lines supports specific recognition of endogenous PPIA and demonstrates the utility of this antibody for western blot applications.

PPIA Antibody Human Mouse WB. Western blot analysis of 1) human T-47D, 2) mouse brain, and 3) mouse bladder lysates using PPIA Antibody demonstrates strong immunoreactive bands at approximately 18 kDa in all samples, consistent with the expected molecular weight of PPIA (Cyclophilin A). PPIA is a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, protein trafficking, and regulation of cellular stress response pathways. The consistent detection of endogenous PPIA across human and mouse tissues highlights its ubiquitous expression and supports the utility of this antibody for cross-species western blot applications. Predicted molecular weight: ~18 kDa. Observed molecular weight: ~18 kDa. A faint higher molecular weight band visible in the mouse bladder sample may represent a post-translationally modified form of PPIA, protein complex formation, or minor nonspecific reactivity.

PPIA Antibody Human Brain IHC. Immunohistochemical staining of FFPE human brain tissue using PPIA Antibody demonstrates widespread cytoplasmic HRP-DAB brown staining throughout neural tissue, with positive immunoreactivity observed in neuronal cell bodies and surrounding neuropil. The staining pattern is consistent with expression of PPIA (Cyclophilin A), a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress response pathways. Robust expression within brain tissue supports the important role of Cyclophilin A in maintaining protein homeostasis and neuronal function within the central nervous system. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

PPIA Antibody WiDr Flow Cytometry. Flow cytometric analysis of human WiDr cells stained with PPIA Antibody demonstrates a clear positive fluorescence shift (green histogram) relative to the isotype control population (blue histogram). The observed increase in fluorescence intensity is consistent with expression of PPIA (Cyclophilin A), a ubiquitously expressed peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress response pathways. The distinct separation between the PPIA antibody-stained and control populations supports specific detection of endogenous Cyclophilin A by flow cytometry and demonstrates the utility of this antibody for FACS applications in human cell lines. Blue = isotype control. Green = PPIA antibody.