POLR2H Antibody / RNA Polymerase II Subunit Antibody (RQ8029)

POLR2H Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells with POLR2H Antibody (red) and alpha Tubulin antibody (green). HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. POLR2H Antibody / RNA Polymerase II Subunit Antibody demonstrates nuclear staining consistent with RNA Polymerase II Subunit H expression and supports studies of transcription machinery, RNA polymerase complex function and gene expression regulation pathways.

POLR2H Antibody HeLa Cell IF. Immunofluorescent staining of human HeLa cells with POLR2H Antibody (green) and alpha Tubulin antibody (red). Enzyme antigen retrieval was performed for 15 minutes, followed by blocking with 10% goat serum. Cells were incubated with rabbit anti-POLR2H antibody at 5 ug/ml and mouse anti-alpha Tubulin antibody overnight at 4°C, followed by Fluoro488 and Cy3 conjugated secondary antibodies. POLR2H Antibody / RNA Polymerase II Subunit Antibody demonstrates nuclear staining consistent with RNA Polymerase II Subunit H expression and supports studies of transcription machinery, RNA polymerase complex activity and gene expression regulation pathways.

POLR2H Antibody Human Lung Cancer IF. Immunofluorescent staining of FFPE human lung cancer tissue with POLR2H Antibody at 5 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-POLR2H antibody overnight at 4°C, followed by Cy3 conjugated goat anti-rabbit IgG secondary antibody (red). Nuclei were counterstained with DAPI (blue). POLR2H Antibody / RNA Polymerase II Subunit Antibody demonstrates nuclear staining consistent with RNA Polymerase II Subunit H expression and supports studies of transcription machinery, RNA polymerase complex activity and gene expression regulation pathways.

POLR2H Antibody MCF-7 Cell IP. Immunoprecipitation of POLR2H protein from 500 ug of human MCF-7 whole cell lysate using 2 ug of POLR2H Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and rabbit IgG control samples included for comparison. A specific band was detected at approximately 17 kDa, consistent with the predicted molecular weight of RNA Polymerase II Subunit H. POLR2H Antibody / RNA Polymerase II Subunit Antibody successfully enriches endogenous POLR2H protein and supports studies of RNA polymerase complex formation, transcription machinery and gene expression regulation pathways.

POLR2H Antibody Human HeLa and MCF7 Cell WB. Western blot testing of human HeLa and MCF-7 cell lysates with POLR2H Antibody. Lane 1: human HeLa; Lane 2: human MCF-7. A specific band was detected at approximately 17 kDa, consistent with the predicted molecular weight of RNA Polymerase II Subunit H. POLR2H Antibody / RNA Polymerase II Subunit Antibody detects endogenous POLR2H expression and supports studies of transcription machinery, RNA polymerase complex function, chromatin-associated regulation and gene expression pathways.

POLR2H Antibody Human, Rat and Mouse WB. Western blot testing of human 293T, HeLa and MCF-7 cell lysates, rat liver tissue lysate, rat RH35 cell lysate, mouse liver tissue lysate and mouse HEPA1-6 cell lysate with POLR2H Antibody at 0.5 ug/ml. Lane 1: human 293T; Lane 2: human HeLa; Lane 3: human MCF-7; Lane 4: rat liver; Lane 5: rat RH35; Lane 6: mouse liver; Lane 7: mouse HEPA1-6. A specific band was detected at approximately 17 kDa, consistent with the predicted molecular weight. POLR2H Antibody / RNA Polymerase II Subunit Antibody detects endogenous RNA Polymerase II Subunit H expression and supports studies of transcription machinery, gene expression regulation and RNA polymerase complex function.