PKC alpha Antibody / PRKCA Polyclonal Antibody (R31731)

PKC alpha Antibody by WB in human and rodent samples. Western blot analysis using PRKCA Polyclonal Antibody demonstrates immunoreactive bands near the predicted molecular weight of approximately 77 kDa. Samples were loaded in the following order: lane 1, rat brain tissue; lane 2, mouse brain tissue; lane 3, mouse heart tissue; and lane 4, human 22Rv1 prostate carcinoma cells. A prominent band is visible near 77 kDa in all four lanes, consistent with detection of PKC alpha. The comparable electrophoretic migration across human, mouse, and rat samples supports the stated cross-species reactivity of this rabbit polyclonal antibody.

PKC alpha Antibody by IHC in human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human breast carcinoma using PRKCA Polyclonal Antibody at 1 ug/ml demonstrates brown immunoreactivity in tumor cells. Staining is predominantly cytoplasmic, with variable intensity among individual cells. Sections underwent heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, for 10-20 minutes before antibody incubation. The observed staining illustrates PKC alpha expression within the malignant epithelial tissue.

PKC alpha Antibody by IHC in mouse intestinal epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse intestine using PRKCA Polyclonal Antibody at 1 ug/ml demonstrates brown immunoreactivity within intestinal epithelial cells. Staining is distributed throughout portions of the villous epithelium, with variable intensity among cells. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6.0, for 10-20 minutes. The staining pattern illustrates PKC alpha expression in mouse intestinal tissue.

PKC alpha Antibody by IHC in rat intestinal epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded rat intestine using PRKCA Polyclonal Antibody at 1 ug/ml demonstrates brown staining in intestinal epithelial cells. Immunoreactivity is visible along the villous epithelium, with prominent cytoplasmic staining in subsets of cells. Sections were subjected to heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, for 10-20 minutes before staining. These findings illustrate detection of PKC alpha in rat intestinal tissue.

PKC alpha Antibody by WB in human cell lines and rodent brain tissue. Western blot analysis using PRKCA Polyclonal Antibody demonstrates immunoreactive bands in human and rodent samples. Lane 1 contains human U87 cell lysate; lane 2, human HEL cell lysate; lane 3, human A549 cell lysate; lane 4, human U2OS cell lysate; lane 5, rat brain lysate; and lane 6, mouse brain lysate. Each lane was loaded with 30 ug of total protein under reducing conditions. Proteins were separated by 5-20% SDS-PAGE and transferred to nitrocellulose. The membrane was incubated overnight at 4oC with the primary antibody at 0.5 ug/ml. Prominent bands migrate near the expected 77 kDa molecular weight, with differences in signal intensity among samples.