- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody [clone ADE-22] (RQ5249)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5249 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 449 |
|
| Availability | 1-2 weeks |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | ADE-22 |
| Purity | Affinity purified |
| UniProt | P08670 |
| Applications | Western Blot : 1:500-1:2000 |
| Limitations | This Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody recognizes vimentin phosphorylated at serine 72, a highly regulated post-translational modification that controls intermediate filament dynamics during mitosis. Vimentin is a type III intermediate filament protein expressed predominantly in mesenchymal cells, where it provides structural support, maintains cellular integrity, and coordinates cytoskeletal organization. Phosphorylation at serine 72 promotes filament disassembly and reorganization, enabling the extensive cytoskeletal remodeling required for chromosome segregation and successful cell division. As a result, Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody is an excellent tool for investigating mitotic progression, kinase signaling, and phosphorylation-dependent regulation of the cytoskeleton.
Vimentin phosphorylation is mediated by mitosis-associated kinases in response to cell cycle progression and growth factor signaling. Modification at serine 72 facilitates reversible intermediate filament remodeling, allowing cells to round up during mitosis, assemble the mitotic spindle, complete cytokinesis, and subsequently re-establish the normal filament network following cell division. Because phosphorylation at this residue is transient and tightly regulated, phospho-serine 72 serves as a valuable marker of actively dividing cells rather than overall vimentin expression. Analysis of phospho-vimentin therefore complements total vimentin detection by distinguishing activated cytoskeletal remodeling from constitutive protein abundance.
Aberrant phosphorylation of vimentin has been associated with cancer progression, epithelial-to-mesenchymal transition, enhanced cellular migration, invasion, fibrosis, and metastatic dissemination. Increased serine 72 phosphorylation reflects activation of signaling pathways that promote cytoskeletal plasticity and tumor cell motility, making this modification an important biomarker for studies of cell cycle regulation, oncogenic kinase signaling, therapeutic response, and mechanisms of tumor progression. Monitoring phospho-vimentin can therefore provide valuable insight into dynamic cellular processes that are not evident from total vimentin expression alone.
NSJ Bioreagents' Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody (clone ADE-22) is a recombinant rabbit monoclonal antibody developed for highly specific detection of vimentin phosphorylated at serine 72. The recombinant format provides excellent lot-to-lot consistency while supporting reliable analysis of mitotic signaling, cytoskeletal remodeling, and phosphorylation-dependent cellular events by western blotting, immunohistochemistry, immunofluorescence, and related phosphoprotein applications.
Explore our Protein Microarray Validated Vimentin Antibody page to learn more about vimentin biology, mesenchymal cell markers, and additional validated products.
Optimal dilution of the Phospho-Vimentin (Ser72) Antibody / Mitotic Marker Antibody should be determined by the researcher.
A synthetic peptide specific to human Vimentin (surrounding phosphorylated serine 72) was used as the immunogen for the Phospho-Vimentin (Ser72) Antibody.
Store the Phospho-Vimentin (Ser72) Antibody at -20oC.
Phospho-Vimentin (Ser72) antibody, Phospho-VIM (Ser72) antibody, Phosphorylated Vimentin Ser72 antibody, Vimentin phospho Ser72 antibody, Vimentin pSer72 antibody, Phospho-Vimentin S72 antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
