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Home >> Antibodies >> Phospho-Syk Antibody (pY525/526)

Phospho-Syk Antibody (pY525/526) (F48459)

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  Catalog No Formulation Size Price (USD)  
Image F48459-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 429
Image
F48459-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 199
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Western blot testing of lysate from human Raji cells that was untreated (-) and treated with 200 nm TPA for 30 min (+).
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Western blot testing of lysate from human Raji cells that was untreated (-) and treated with 200 nm TPA for 30 min (+).
IHC analysis of FFPE human hepatocarcinoma tissue stained with the phospho-Syk antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P43405
Applications Western Blot : 1:100-1:250
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This phospho-Syk antibody is available for research use only.
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Description

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Application Notes

Titration of the phospho-Syk antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-Syk antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to the amino acid residues surrounding pY525/526 of human Syk.

Storage

Aliquot the phospho-Syk antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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