Phospho-KLF4 (S245) Antibody / Phosphorylated KLF4 Antibody (F48604)

Phospho-KLF4 (S245) Antibody Peptide Specificity Dot Blot. Dot blot analysis demonstrating the specificity of Phospho-KLF4 (S245) Antibody using 50 ng of phosphorylated or non-phosphorylated KLF4 peptide per dot. Strong immunoreactivity was observed with the phosphorylated peptide, while no detectable binding was seen with the corresponding non-phosphorylated peptide, confirming phosphorylation-dependent recognition of KLF4 at serine 245. This Phospho-KLF4 (S245) Antibody is suitable for studies of KLF4 phosphorylation, kinase signaling, post-translational regulation, and transcription factor activation.

Phospho-KLF4 (S245) Antibody HeLa Cell IF. Immunofluorescent staining of fixed and permeabilized human HeLa cells using Phospho-KLF4 (S245) Antibody at a 1:200 dilution. Phosphorylated KLF4 immunoreactivity (green) is specifically localized to the nuclei, with minimal staining in the nucleoli or cytoplasm, consistent with phosphorylation of the active nuclear transcription factor. The actin cytoskeleton is shown in red to provide cellular context. This Phospho-KLF4 (S245) Antibody is suitable for studies of KLF4 phosphorylation, nuclear signaling, transcription factor regulation, and kinase-mediated cellular signaling.