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Home >> Antibodies >> Phospho-KLF4 Antibody (pS245)

Phospho-KLF4 Antibody (pS245) (F48604)

  Catalog No Formulation Size Price (USD)  
Image F48604-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F48604-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Dot blot analysis of phospho-KLF4 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Fluorescent confocal image of HeLa cells stained with phospho-KLF4 antibody at 1:200. Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the nucleoli or the cytoplasm.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O43474
Applications Immunofluorescence : 1:10-1:50
Dot blot : 1:500
Limitations This phospho-KLF4 antibody is available for research use only.
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Description

Krueppel-like factor family members are involved in growth regulation and a wide range of developmental processes. KLF4 is highly expressed in epithelial tissues including skin and stomach/intestine. Studies have shown that it can function as both an oncogene, upregulating hSSC, and a tumor suppressor, down-regulating colorectal cancer.

Application Notes

Titration of the phospho-KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-KLF4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS245 of human KLF4.

Storage

Aliquot the phospho-KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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