PHEX Antibody / Phosphate Regulating Endopeptidase Antibody (RQ5999)

PHEX Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using PHEX Antibody / Phosphate Regulating Endopeptidase Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with PHEX Antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen for visualization. PHEX exhibits predominantly membranous and cytoplasmic staining in tumor cells, consistent with its localization as a membrane-associated metalloprotease involved in phosphate homeostasis and FGF23 regulation. This PHEX Antibody, also called Phosphate Regulating Endopeptidase Antibody, is suitable for studies of phosphate metabolism, bone mineralization, and X-linked hypophosphatemia.

PHEX Antibody Rat Knee Joint IHC. Immunohistochemical staining of FFPE rat knee joint tissue using PHEX Antibody / Phosphate Regulating Endopeptidase Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with PHEX Antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen for visualization. PHEX staining is observed in cells associated with mineralized skeletal tissue, consistent with its established role in regulating phosphate homeostasis, FGF23 signaling, and bone mineralization. This PHEX Antibody, also called Phosphate Regulating Endopeptidase Antibody, is suitable for studies of skeletal biology, phosphate metabolism, and X-linked hypophosphatemia.

PHEX Antibody Human Cancer Cell WB. Western blot analysis of human PC-3 (lane 1), U251 (lane 2), A431 (lane 3), and A549 (lane 4) whole cell lysates using PHEX Antibody / Phosphate Regulating Endopeptidase Antibody at 0.5 ug/ml. A prominent band is detected at approximately 100-110 kDa in all samples, which is consistent with the extensively glycosylated mature form of PHEX. Although the calculated molecular weight is approximately 86 kDa, glycosylation is known to produce an apparent molecular weight shift into the 95-110 kDa range. This PHEX Antibody, also called Phosphate Regulating Endopeptidase Antibody, is suitable for studies of phosphate homeostasis, FGF23 signaling, bone mineralization, and X-linked hypophosphatemia.

PHEX Antibody Human PC-3 Cell FACS. Flow cytometry analysis of fixed human PC-3 cells stained with PHEX Antibody / Phosphate Regulating Endopeptidase Antibody (blue). Cells were blocked with normal goat serum and incubated with PHEX Antibody at 1 ug/10^6 cells, followed by a Fluoro488-conjugated goat anti-rabbit secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the PHEX-positive population demonstrates specific detection of PHEX expression. This PHEX Antibody, also called Phosphate Regulating Endopeptidase Antibody, is suitable for flow cytometric analysis of PHEX expression and studies of phosphate homeostasis, FGF23 signaling, and bone mineralization.