PDHX Antibody / Pyruvate Dehydrogenase Protein X Antibody (F47892)

PDHX Antibody Detection in Human SH-SY5Y Neuroblastoma Cells by IF. Immunofluorescence staining of human SH-SY5Y neuroblastoma cells with PDHX Antibody shows green immunoreactivity concentrated in a punctate and perinuclear cytoplasmic distribution. This pattern is consistent with the mitochondrial localization of pyruvate dehydrogenase protein X rather than the nuclear localization stated in the previous figure description. The fluorescence demonstrates cellular detection of PDHX, also known as E3-binding protein, using this Pyruvate Dehydrogenase Protein X Antibody.

PDHX Antibody Detection in Human NCI-H460 Lung Cancer Cells by WB. Western blot testing of human NCI-H460 lung cancer cell lysate with PDHX Antibody shows a prominent discrete band migrating at approximately 40-42 kDa. This is below the approximately 54 kDa calculated molecular weight of canonical human PDHX. Because the observed migration differs substantially from the calculated mass, I would not describe this band as the expected full-length protein without additional supporting evidence. The experiment demonstrates antibody reactivity with a discrete protein in NCI-H460 lysate using this Pyruvate Dehydrogenase Protein X Antibody.

PDHX Antibody Detection of Transfected Human HEK293 Cells by WB. Western blot analysis of human HEK293 cells with PDHX Antibody shows little or no corresponding signal in non-transfected cells (lane 1) and a strong band at approximately 42-45 kDa following PDHX transfection (lane 2). The marked increase in signal in the transfected sample provides useful support for recognition of the PDHX gene product, historically also called PDX1. The observed band migrates below the approximately 54 kDa calculated mass of canonical PDHX, so the figure should not be described as showing the expected molecular weight without further characterization.

PDHX Antibody Staining in Human Hepatocellular Carcinoma by IHC. Immunohistochemistry staining of FFPE human hepatocellular carcinoma tissue with PDHX Antibody shows widespread granular brown cytoplasmic immunoreactivity within tumor cells, while nuclei are predominantly unstained. The cytoplasmic staining pattern is compatible with the mitochondrial localization of pyruvate dehydrogenase protein X and contrasts with the nuclear localization expected for the unrelated pancreatic transcription factor that also uses the PDX1 designation. The staining demonstrates detection of PDHX in human hepatocellular carcinoma tissue using this Pyruvate Dehydrogenase Protein X Antibody.