• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> PDGFRA Antibody / Platelet Derived Growth Factor Receptor alpha

PDGFRA Antibody / Platelet Derived Growth Factor Receptor alpha [clone PDGFRA/7407] (V5049)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V5049-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
Image
V5049-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
Image
V5049SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Bulk quote request
›
PDGFRA Antibody Colon Cancer IHC. Immunohistochemistry staining of FFPE human colon cancer tissue using PDGFRA Antibody / Receptor Tyrosine Kinase Antibody (clone PDGFRA/7407) demonstrates membranous and cytoplasmic HRP-DAB brown staining within malignant gland-forming epithelial cells. The staining pattern is consistent with expression of PDGFRA, a receptor tyrosine kinase that mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival. PDGFRA contributes to growth factor-dependent communication within the tumor microenvironment and has been widely studied in cancer biology due to its roles in cellular growth regulation and tissue remodeling. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to immunostaining.
SDS-PAGE analysis of purified, BSA-free PDGFRA antibody (clone PDGFRA/7407) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PDGFRA/7407
Purity Protein A/G affinity
UniProt P16234
Localization Membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This PDGFRA antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

PDGFRA Antibody specifically detects Platelet Derived Growth Factor Receptor alpha. Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-a and -beta, which are endowed with protein tyrosine kinase domains. PDGFR-a can bind to both A and B subunits of PDGF, while PDGFR-beta can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two a receptors, PDGF-AB induces dimerization of aa and abeta and PDGF-BB induces the formation of three types of dimers, aa, abeta and betabeta. Translocation of the PDGFR-beta gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.

Learn more about PDGFRA expression, platelet-derived growth factor signaling, mesenchymal development, and receptor tyrosine kinase biology on our PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody page.

Application Notes

Optimal dilution of the PDGFRA antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the PDGFRA antibody.

Storage

Aliquot the PDGFRA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all PDGFR-A antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.