PDE5A Antibody / cGMP Specific Phosphodiesterase Antibody (R32652)

PDE5A Antibody Human Thyroid Cancer IHC. Immunohistochemistry was performed on paraffin-embedded human thyroid cancer tissue using PDE5A Antibody at 2 ug/mL. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer, and the tissue was incubated with the primary antibody overnight at 4°C. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Distinct cytoplasmic staining is observed in the thyroid carcinoma cells, consistent with PDE5A expression, while surrounding stromal elements exhibit comparatively low background staining. These results demonstrate that this cGMP Specific Phosphodiesterase Antibody is suitable for immunohistochemical detection of PDE5A in formalin-fixed, paraffin-embedded human thyroid cancer tissue.

PDE5A Antibody Mouse and Rat Lung WB. Western blot analysis was performed using PDE5A Antibody at 0.5 ug/mL. Lane 1: rat lung tissue lysate; lane 2: mouse lung tissue lysate. Proteins were separated on an 8% SDS-PAGE gel, transferred to a nitrocellulose membrane, and the blot was incubated with the primary antibody overnight at 4°C. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody and enhanced chemiluminescence. A specific immunoreactive band is detected at approximately 100 kDa in both rat and mouse lung lysates, consistent with the expected molecular weight of PDE5A. These results demonstrate that this cGMP Specific Phosphodiesterase Antibody is suitable for Western blot detection of PDE5A in rodent tissues.