Phosphodiesterase 5A Antibody / cGMP Binding Phosphodiesterase Antibody (R34720)

Phosphodiesterase 5A Antibody Human Lung WB. Western blot analysis was performed using Phosphodiesterase 5A Antibody at 0.3 ug/ml on human lung lysate. A specific immunoreactive band is detected at approximately 100 kDa, consistent with the predicted molecular weight of the PDE5A isoforms (~100/95 kDa for isoforms 1 and 2). The clean, specific band demonstrates that this cGMP Binding Phosphodiesterase Antibody is suitable for Western blot detection of PDE5A in human tissue.

Phosphodiesterase 5A Antibody Human HEK293 Cell WB. Western blot analysis was performed using lysates from human HEK293 cells mock transfected (lane 1) or transfected to overexpress PDE5A (lane 2). Phosphodiesterase 5A Antibody was used at 0.3 ug/ml. A strong immunoreactive band is detected at approximately 100 kDa exclusively in the PDE5A-overexpressing sample, consistent with the predicted molecular weight of PDE5A isoforms (~100/95 kDa for isoforms 1 and 2), while the mock-transfected control shows no detectable signal. These results demonstrate that this cGMP Binding Phosphodiesterase Antibody specifically detects PDE5A by Western blot.

Phosphodiesterase 5A Antibody HeLa Cell IF. Immunofluorescence staining was performed on human HeLa cells using Phosphodiesterase 5A Antibody at 10 ug/ml. Bound antibody was detected with a fluorophore-conjugated secondary antibody (green), and nuclei were counterstained with DAPI (blue). The merged image demonstrates strong cytoplasmic staining with minimal nuclear signal, while the negative control shows no specific fluorescence. These results demonstrate that this cGMP Binding Phosphodiesterase Antibody is suitable for immunofluorescent detection of PDE5A in cultured human cells.

Phosphodiesterase 5A Antibody HEK293 Cell FACS. Flow cytometric analysis was performed on human HEK293 cells using Phosphodiesterase 5A Antibody at 10 ug/ml. The black histogram represents the isotype control, while the blue histogram represents cells stained with the PDE5A antibody. A clear rightward shift of the blue population relative to the isotype control demonstrates specific antibody binding and confirms PDE5A expression in HEK293 cells. These results demonstrate that this cGMP Binding Phosphodiesterase Antibody is suitable for flow cytometric detection of PDE5A.