PD-L1 Antibody / Tumor Microenvironment Immune Checkpoint Antibody [clone PDL1/2746] (V3955)

PD-L1 Antibody Cervical Carcinoma IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human cervical carcinoma using clone PDL1/2746. The rabbit monoclonal antibody demonstrates prominent membranous HRP-DAB brown staining in tumor epithelial cells, with additional staining observed in scattered tumor-associated immune cells, highlighting immune checkpoint activity within the tumor microenvironment. The staining pattern reflects heterogeneous PD-L1 expression across tumor regions and supports evaluation of tumor-immune interactions and checkpoint signaling. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

PD-L1 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human breast carcinoma using clone PDL1/2746. The antibody demonstrates membranous HRP-DAB brown staining in tumor epithelial cells with additional signal in tumor-associated immune cells, highlighting immune checkpoint activity within the tumor microenvironment. The staining pattern shows heterogeneous expression across tumor regions, supporting evaluation of tumor-immune interactions and PD-L1-associated signaling. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

PD-L1 Antibody Lung Squamous Cell Carcinoma IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human lung squamous cell carcinoma using clone PDL1/2746. The antibody demonstrates strong membranous HRP-DAB brown staining in tumor epithelial cells, with additional signal in tumor-associated immune cells, reflecting active immune checkpoint signaling within the tumor microenvironment. The staining pattern shows regionally heterogeneous expression across tumor nests, supporting evaluation of PD-L1 distribution and tumor-immune interactions. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

PD-L1 Antibody Basal Cell Carcinoma IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human basal cell carcinoma using clone PDL1/2746. The antibody demonstrates membranous HRP-DAB brown staining in tumor epithelial cells, with focal staining in tumor-associated immune cells within the surrounding stroma, consistent with localized immune checkpoint activity. The staining pattern highlights heterogeneous PD-L1 expression across tumor regions, supporting evaluation of tumor-immune interactions and checkpoint signaling dynamics. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

PD-L1 Antibody Jurkat Cells IF. Immunofluorescence analysis of PD-L1 (CD274) expression in human Jurkat cells using clone PDL1/2746. The antibody (green) demonstrates cell-associated staining consistent with PD-L1 localization, while nuclei are counterstained with Reddot (red). The staining pattern supports detection of CD274 in immune cell populations and enables visualization of expression at the single-cell level.

PD-L1 Antibody Western Blot. Western blot analysis of PD-L1 (CD274) expression in human and mouse cell lysates including RAW, A549, THP-1, MOLT-4, Raji, and MDA-MB-231 using clone PDL1/2746. A band is detected at approximately 45–55 kDa, consistent with glycosylated PD-L1, with possible lower signal near the predicted molecular weight of ~34 kDa representing the unglycosylated form. PD-L1 is a known glycoprotein, and the observed band pattern reflects variable glycosylation states across different cell types, supporting expected expression and post-translational modification of CD274.

Western blot testing of human cell lysates with PD-L1 antibody (PDL1/2746). Expected molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

PD-L1 Antibody Jurkat Cells FACS. Flow cytometry analysis of PD-L1 (CD274) expression in fixed and permeabilized human Jurkat cells using clone PDL1/2746. The PD-L1 antibody (orange) demonstrates a clear right-shifted population compared to cells alone (blue) and isotype control (red), indicating specific detection of CD274. The distinct separation between negative and positive populations supports accurate gating and reliable identification of PD-L1-expressing cells in flow cytometry assays.

PD-L1 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PD-L1 antibody (clone PDL1/2746). These results demonstrate the foremost specificity of the PDL1/2746 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2746) as confirmation of integrity and purity.