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Home >> Antibodies >> PCNA Antibody

PCNA Antibody (F48249)

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  Catalog No Formulation Size Price (USD)  
Image F48249-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48249-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate. Expected molecular weight: 29-36 kDa.
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Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate. Expected molecular weight: 29-36 kDa.
Western blot analysis of PCNA antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the PCNA gene.
IHC analysis of FFPE human hepatocarcinoma tissue stained with PCNA antibody
Confocal immunofluorescent analysis of PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of PCNA antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
PCNA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Rat, Bovine, Primate, Hamster
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P12004
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This PCNA antibody is available for research use only.
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Description

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Application Notes

Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 231-261 from the human protein was used as the immunogen for this PCNA antibody.

Storage

Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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