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Home >> Antibodies >> PBX1 Antibody

PBX1 Antibody [clone PCRP-PBX1-3C8] (V8884)

  Catalog No Formulation Size Price (USD)  
Image V8884-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8884-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8884SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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FACS staining of PFA-fixed human HeLa cells with PBX1 antibody (blue, clone PCRP-PBX1-3C8) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free PBX1 antibody (PCRP-PBX1-3C8) as confirmation of integrity and purity.
Immunofluorescent staining of PFA-fixed human HeLa cells using PBX1 antibody (green, clone PCRP-PBX1-3C8) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PBX1 antibody (clone PCRP-PBX1-3C8). These results demonstrate the foremost specificity of the PCRP-PBX1-3C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name PCRP-PBX1-3C8
Purity Protein A/G affinity
UniProt P40424
Localization Nucleus, cytoplasm
Applications ELISA (order BSA-free format for coating) :
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This PBX1 antibody is available for research use only.
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Description

Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1.

Application Notes

Optimal dilution of the PBX1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PBX1 protein was used as the immunogen for the PBX1 antibody.

Storage

Aliquot the PBX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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