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Home >> Antibodies >> PAX4 Antibody

PAX4 Antibody (RQ5748)

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  Catalog No Formulation Size Price (USD)  
Image RQ5748 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human A431 cells with PAX4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX4 antibody.
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Flow cytometry testing of human A431 cells with PAX4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX4 antibody.
Western blot testing of 1) human HL60, 2) rat thymus and 3) mouse thymus tissue lysate. Predicted molecular weight ~38 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O43316
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PAX4 antibody is available for research use only.
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Description

Paired box gene 4, also known as PAX4, is a protein which in humans is encoded by the PAX4 gene. This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.

Application Notes

Optimal dilution of the PAX4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K76-P350) was used as the immunogen for the PAX4 antibody.

Storage

After reconstitution, the PAX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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