p63 Antibody / Basal Cell Marker Antibody [clone TP63/2428] (V3948)

p63 Antibody. Immunohistochemistry analysis of Tumor protein 63 (TP63) in FFPE human basal cell carcinoma tissue using a p63 antibody as a basal cell marker (clone TP63/2428) demonstrates strong HRP-DAB brown nuclear staining in tumor cells with basal-like morphology. The staining is diffuse and nuclear, highlighting the expansion of TP63-positive basal cell populations within the tumor and supporting its identity as a basal cell-derived carcinoma. Surrounding stromal components remain negative, providing clear contrast and reinforcing the lineage-specific expression pattern. The crisp nuclear localization and widespread tumor cell positivity are consistent with the role of TP63 in basal cell maintenance and epithelial proliferation. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

IHC testing of FFPE human prostate carcinoma with p63 antibody (clone TP63/2428). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

p63 Antibody protein microarray specificity analysis using a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding of clone TP63/2428 to Tumor protein 63 (TP63). The antibody shows a dominant top-ranked signal for TP63 with a high Z-score and clear separation from all other proteins on the array, indicating strong target recognition with minimal off-target interaction. Signals for related proteins, including TP73L family members, are substantially lower, supporting specificity within closely related transcription factor families. Z-score reflects signal intensity relative to the array mean, while S-score represents the separation between TP63 and the next highest-ranking proteins, confirming clear discrimination of the intended target. These results support the use of this p63 antibody as a basal cell marker in applications requiring high specificity and confident interpretation of TP63 detection.

SDS-PAGE analysis of purified, BSA-free p63 antibody (clone TP63/2428) as confirmation of integrity and purity.

Flow cytometry testing of PFA-fixed human HeLa cells with p63 antibody (clone TP63/2428); Red=isotype control, Blue= p63 antibody.