p40 Antibody / Squamous Lineage Determination Marker Antibody [clone P40/8225R] (V5028)

p40 Antibody / Squamous Lineage Determination Marker Antibody. Immunohistochemistry analysis of Tumor protein p40 (deltaNp63) in FFPE human prostate carcinoma tissue using a p40 antibody (clone P40/8225R) demonstrates distinct HRP-DAB brown nuclear staining in basal epithelial cells outlining residual benign glandular structures, while malignant luminal tumor cells remain negative. The nuclear-restricted staining pattern highlights preserved basal cell layers and clearly contrasts with carcinoma regions lacking basal cells, supporting interpretation of epithelial lineage and glandular architecture. Surrounding stromal cells show minimal staining, providing clean background and strong signal specificity. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

p40 Antibody. Immunohistochemistry analysis of FFPE human lung carcinoma tissue demonstrates strong HRP-DAB brown nuclear staining in tumor cell clusters consistent with squamous differentiation, while surrounding stromal cells remain negative. The nuclear-restricted staining pattern supports use of this antibody as a squamous lineage determination marker, highlighting epithelial tumor cells and enabling clear distinction from non-squamous components. Staining is intense and well-defined, allowing accurate interpretation of tumor phenotype and cellular organization. Expression is consistent with deltaNp63, a TP63 isoform associated with squamous epithelial lineage commitment. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free p40 antibody (clone P40/8225R) as confirmation of integrity and purity.