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Home >> Antibodies >> OPLAH Antibody / 5-oxo-L-prolinase / 5-OPase

OPLAH Antibody / 5-oxo-L-prolinase / 5-OPase (RQ7944)

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  Catalog No Formulation Size Price (USD)  
Image RQ7944 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human PC-3 cells with OPLAH antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human PC-3 cells with OPLAH antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human T-47D, 3) monkey COS-7, 4) rat liver, 5) rat brain, 6) mouse liver and 7) mouse brain tissue lysate with OPLAH antibody. Predicted molecular weight ~137 kDa.
Flow cytometry testing of human HEL cells with OPLAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPLAH antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O14841
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This OPLAH antibody is available for research use only.
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Description

The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).

Application Notes

Optimal dilution of the OPLAH antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H788-E1072) was used as the immunogen for the OPLAH antibody.

Storage

After reconstitution, the OPLAH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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