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Home >> Antibodies >> NSFL1C Antibody / NSFL1 cofactor p47

NSFL1C Antibody / NSFL1 cofactor p47 (RQ8636)

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  Catalog No Formulation Size Price (USD)  
Image RQ8636 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A549 cells with NSFL1C antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with NSFL1C antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Jurkat, 2) human PC-3, 3) rat RH35 and 4) mouse NIH 3T3 cell lysate with NSFL1C antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with NSFL1C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NSFL1C antibody.
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UNZ2
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This NSFL1C antibody is available for research use only.
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Description

NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene. N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Application Notes

Optimal dilution of the NSFL1C antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids E20-E291) was used as the immunogen for the NSFL1C antibody.

Storage

After reconstitution, the NSFL1C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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