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Home >> Antibodies >> NSE Antibody / Neuron Specific Enolase / ENO2

NSE Antibody / Neuron Specific Enolase / ENO2 [clone ENO/8253R] (V4230)

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  Catalog No Formulation Size Price (USD)  
Image V4230-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4230-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4230SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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IHC staining of FFPE human pancreas tissue with NSE antibody (clone ENO/8253R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free NSE gamma antibody (clone ENO/8253R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ENO/8253R
Purity Protein A/G affinity
UniProt P09104
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This NSE antibody is available for research use only.
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Description

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It is usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

For detection of neuron-specific enolase (NSE), also known as gamma enolase, across tissue types, see our NSE antibody.

Application Notes

Optimal dilution of the NSE antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human NSE gamma (within amino acids 234-434) was used as the immunogen for the NSE antibody.

Storage

Aliquot the NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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