NPPA Antibody / Atrial Natriuretic Peptide/Factor Antibody (F54517)

NPPA Antibody Human Prostate Carcinoma IHC. Immunohistochemistry staining of FFPE human prostate carcinoma tissue using NPPA antibody demonstrates predominantly cytoplasmic staining within gland-forming epithelial tumor cells, consistent with expression of Natriuretic Peptide Precursor A (NPPA), the precursor of Atrial Natriuretic Peptide (ANP/ANF). NPPA is a peptide hormone precursor that participates in endocrine signaling pathways regulating cardiovascular homeostasis, fluid balance, and intercellular communication. The observed epithelial staining pattern supports the utility of NPPA antibody for studies of peptide hormone expression, endocrine signaling networks, and natriuretic peptide biology in normal and neoplastic tissues. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

NPPA Antibody RD Cell Flow Cytometry. Flow cytometric analysis of fixed and permeabilized human RD cells stained with NPPA antibody demonstrates a pronounced rightward shift of the green histogram (NPPA antibody) relative to the blue histogram (isotype control), consistent with expression of Natriuretic Peptide Precursor A (NPPA). NPPA encodes the precursor protein for Atrial Natriuretic Peptide (ANP/ANF), a peptide hormone involved in cardiovascular homeostasis, endocrine signaling, and regulation of fluid balance. The distinct separation between the NPPA-stained and control populations supports specific detection of intracellular NPPA and demonstrates the utility of this antibody for flow cytometric studies of natriuretic peptide biology and hormone-related signaling pathways. Cells were fixed, permeabilized, and stained prior to analysis.

NPPA Antibody Human Fetal Heart and Mouse Heart WB. Western blot analysis of human fetal heart and mouse heart tissue lysates using NPPA antibody demonstrates distinct immunoreactive bands at approximately 17 kDa in both samples, consistent with the expected molecular weight of Natriuretic Peptide Precursor A (NPPA). NPPA is the precursor protein for Atrial Natriuretic Peptide (ANP/ANF), a cardiac-derived hormone that regulates cardiovascular homeostasis, fluid balance, and endocrine signaling pathways. The conserved detection of NPPA in both human and mouse cardiac tissues supports the utility of this antibody for studies of cardiac endocrine biology, natriuretic peptide signaling, and cardiovascular regulation. Predicted molecular weight: ~17 kDa.