NOTCH1 Antibody / Cell Fate Determination Receptor [clone BGA-14] (RQ6458)

NOTCH1 Antibody HeLa IF. Immunofluorescent staining of human HeLa cells using NOTCH1 antibody demonstrates prominent cytoplasmic and membranous green fluorescence, consistent with expression of NOTCH1, a transmembrane receptor that serves as a central regulator of the Notch signaling pathway. NOTCH1 mediates cell-to-cell communication and controls transcriptional programs involved in cell fate determination, differentiation, proliferation, and tissue development. The observed staining pattern is consistent with the expected cellular localization of NOTCH1 and supports the utility of this antibody for studies of developmental signaling, stem cell biology, signal transduction, and cellular communication pathways. Nuclei are visualized with DAPI nuclear stain (blue).

NOTCH1 Antibody Human Liver IHC. Immunohistochemistry staining of FFPE human liver tissue using NOTCH1 antibody demonstrates diffuse cytoplasmic and membranous staining within hepatocyte populations, consistent with expression of NOTCH1, a transmembrane receptor that functions as a central regulator of the Notch signaling pathway. NOTCH1 mediates cell-to-cell communication and influences transcriptional programs involved in cell fate determination, differentiation, tissue homeostasis, and regenerative responses. The observed staining pattern is consistent with the established role of NOTCH1 in maintaining cellular signaling networks that regulate tissue organization and liver physiology. These findings support the utility of NOTCH1 antibody for studies of developmental signaling, tissue regeneration, and signal transduction pathways in human tissues.

NOTCH1 Antibody Multi-Species WB. Western blot analysis of human HEK293, K562, U-2 OS, Jurkat, HeLa, ThP-1, and Caco-2 cell lysates, together with rat brain, rat PC-12, mouse lung, and mouse brain tissue lysates using NOTCH1 antibody demonstrates a prominent band at approximately 120 kDa across multiple human, rat, and mouse samples. This band is consistent with the transmembrane fragment of NOTCH1 generated during normal receptor processing and activation. NOTCH1 is a transmembrane receptor that functions as a central regulator of the Notch signaling pathway, controlling cell fate determination, differentiation, and developmental signaling through cell-to-cell communication. The broad expression observed across diverse cell lines and tissues is consistent with the widespread biologic importance of NOTCH1 in tissue homeostasis, developmental regulation, and signal transduction. Expected molecular weights: approximately 270 kDa for full-length NOTCH1 and 120 kDa for the transmembrane fragment.