NGF Antibody / Neurotrophin Signaling Marker Antibody [clone BDE-14] (RQ4871)

NGF Antibody SH-SY5Y IF. Immunofluorescence analysis of human SH-SY5Y neuroblastoma cells using recombinant rabbit monoclonal clone BDE-14. NGF / Nerve growth factor staining (green) shows punctate cytoplasmic and perinuclear localization consistent with intracellular neurotrophin processing and secretory vesicle distribution, while nuclei are counterstained with DAPI (blue). The staining pattern aligns with the neuronal-associated biology of SH-SY5Y cells commonly used in neurodevelopment and neurodegeneration research.

NGF Antibody HeLa IF. Immunofluorescence analysis of human HeLa cells using recombinant rabbit monoclonal clone BDE-14 at 1:50 dilution. NGF / Nerve growth factor staining (green) demonstrates diffuse and punctate cytoplasmic localization consistent with intracellular neurotrophin-associated signaling and secretory pathway distribution. Phalloidin-TRITC actin cytoskeleton stain is shown in red and DAPI nuclear counterstain is shown in blue. Merged imaging highlights NGF expression patterns in cultured cervical carcinoma cells.

NGF Antibody Human HeLa Cell IF. Immunofluorescence analysis of human HeLa cells using recombinant rabbit monoclonal clone BDE-14 at 1:150 dilution. NGF / Nerve growth factor staining (green) demonstrates preserved cytoplasmic localization with lower overall signal intensity compared to higher antibody concentration testing, while maintaining distinct intracellular neurotrophin-associated staining patterns. Phalloidin-TRITC actin cytoskeleton stain is shown in red and DAPI nuclear counterstain is shown in blue. Merged imaging supports specific NGF detection in cultured cervical carcinoma cells under reduced antibody concentration conditions.

NGF Antibody Brain Tissue WB. Western blot analysis of rat brain and mouse brain tissue lysates using recombinant rabbit monoclonal NGF antibody clone BDE-14. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. A band is detected at approximately 35 kDa, above the predicted molecular weight of mature NGF, which is commonly reported near 27 kDa depending on precursor processing, dimerization state, and post-translational modification. The observed signal is consistent with the complex biology of Nerve growth factor, including proNGF-associated forms and neurotrophin processing intermediates present in neural tissue.

NGF Antibody Cell Lysate WB. Western blot analysis of human HeLa, 293, and MCF-7 cell lysates using recombinant rabbit monoclonal clone BDE-14 at 1:500 dilution for 1 hour at room temperature. Lane 1: HeLa cell lysate, Lane 2: 293 cell lysate, Lane 3: MCF-7 cell lysate. Prominent bands are detected near 30-35 kDa, consistent with reported NGF / Nerve growth factor precursor-associated and processed neurotrophin forms. Additional higher molecular weight signal in 293 cells and multiple bands in MCF-7 cells may reflect differential precursor processing, post-translational modification, or cell type-specific neurotrophin-associated protein complexes.

NGF Antibody WB. Western blot testing of 1) mouse thyroid and 2) human HeLa lysate with NGF Antibody / Neurotrophin Signaling Marker Antibody. Predicted molecular weight ~27 kDa (dimer).

NGF Antibody Kidney IHC. Immunohistochemistry staining of FFPE human kidney tissue with NGF Antibody / Neurotrophin Signaling Marker Antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.