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Home >> Antibodies >> NEMF Antibody / SDCCAG1

NEMF Antibody / SDCCAG1 (RQ8180)

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  Catalog No Formulation Size Price (USD)  
Image RQ8180 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with NEMF antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with NEMF antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) Jurkat and 3) PC-3 cell lysate with NEMF antibody. Predicted molecular weight ~123 kDa, commonly observed at 123-140 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O60524
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This NEMF antibody is available for research use only.
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Description

Nuclear export mediator factor (NEMF), also called Serologically defined colon cancer antigen 1 (SDCCAG1), is a protein that in humans is encoded by the NEMF gene. This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor.

Application Notes

Optimal dilution of the NEMF antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H81-R607) was used as the immunogen for the NEMF antibody.

Storage

After reconstitution, the NEMF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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