Nebulette Antibody / NEBL Polyclonal Antibody (RQ8188)

Nebulette Antibody by IHC in mouse heart tissue. Immunohistochemistry of FFPE mouse heart tissue using Nebulette Antibody. Brown DAB staining is visible along cardiac muscle fibers, with a distinct striated pattern corresponding to the organization of the contractile apparatus. Hematoxylin counterstaining identifies blue-purple nuclei between the muscle fibers. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0) for 20 minutes. This NEBL Polyclonal Antibody demonstrates immunoreactivity associated with the organized myofibrillar structures of cardiac muscle.

Nebulette Antibody by IHC in rat heart tissue. Immunohistochemistry of FFPE rat heart tissue using Nebulette Antibody. Brown DAB immunoreactivity follows the longitudinal arrangement of cardiac muscle fibers, with visible transverse striations in portions of the tissue. Staining intensity varies among individual fibers, while hematoxylin counterstaining highlights elongated blue-purple nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0) for 20 minutes. This NEBL Polyclonal Antibody demonstrates staining associated with cardiac myofibrils, consistent with the structural role of nebulette in the sarcomere.

Nebulette Antibody by IHC in human heart tissue. Immunohistochemistry of FFPE human heart tissue using Nebulette Antibody at 2 ug/ml. Brown DAB immunoreactivity is distributed throughout cardiac muscle fibers, with prominent cytoplasmic staining and variable intensity among cardiomyocytes. Hematoxylin counterstaining highlights blue nuclei, while connective tissue spaces remain comparatively lightly stained. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4oC. This NEBL Polyclonal Antibody demonstrates immunoreactivity in cardiac muscle, consistent with the tissue distribution of nebulette.

Nebulette Antibody by WB in rat and mouse heart tissue. Western blot analysis of rat and mouse heart lysates using Nebulette Antibody at 0.25 ug/ml. Lane 1: rat heart; lane 2: mouse heart. Each lane contained 30 ug of protein separated by 8% SDS-PAGE under reducing conditions. Strong immunoreactive bands appear near 126 kDa in both samples, slightly above the calculated 116.5 kDa molecular weight of full-length nebulette. Additional lower-molecular-weight bands are prominent in rat heart, particularly between approximately 65 and 105 kDa, while mouse heart shows fewer secondary bands. This NEBL Polyclonal Antibody detects a major protein species consistent with cardiac nebulette.

Nebulette Antibody by WB in human cells and mouse lung. Western blot analysis using Nebulette Antibody at 0.25 ug/ml. Lane 1: SH-SY5Y; lane 2: MCF7; lane 3: mouse lung. Samples were separated by 8% SDS-PAGE under reducing conditions, with 30 ug loaded per lane. A prominent immunoreactive band appears near 35 kDa in all three samples, substantially below the calculated 116.5 kDa molecular weight of full-length nebulette. The identity of this lower-molecular-weight species remains unconfirmed. This NEBL Polyclonal Antibody recognizes an approximately 35 kDa protein under the tested conditions.