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Home >> Antibodies >> NADH-ubiquinone oxidoreductase chain 5 Antibody / MT-ND5

NADH-ubiquinone oxidoreductase chain 5 Antibody / MT-ND5 (F54903)

  Catalog No Formulation Size Price (USD)  
Image F54903-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54903-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human liver tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) CCRF-CEM and 2) Jurkat cell lysate with NADH-ubiquinone oxidoreductase chain 5 antibody. Expected molecular weight: 65-70 kDa.
Flow cytometry testing of human CCRF-CEM cells with NADH-ubiquinone oxidoreductase chain 5 antibody; Blue=isotype control, Green= NADH-ubiquinone oxidoreductase chain 5 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P03915
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:100
Limitations This NADH-ubiquinone oxidoreductase chain 5 antibody is available for research use only.
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Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Application Notes

The stated application concentrations are suggested starting points. Titration of the NADH-ubiquinone oxidoreductase chain 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 544-570 from the human protein was used as the immunogen for the NADH-ubiquinone oxidoreductase chain 5 antibody.

Storage

Aliquot the NADH-ubiquinone oxidoreductase chain 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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