MYL1 Antibody / Fast Skeletal Muscle Myosin Light Chain Marker / Center Region (F54184)

MYL1 Antibody Multi-Sample WB. Western blot analysis of human and rodent lysates using MYL1 antibody detecting Myosin light chain 1. Lane 1: human heart, Lane 2: human skeletal muscle, Lane 3: mouse skeletal muscle, Lane 4: rat skeletal muscle. A band is detected at approximately 20-22 kDa, consistent with the predicted molecular weight of MYL1. Stronger signal in skeletal muscle samples compared to heart tissue aligns with the preferential expression of MYL1 in fast skeletal muscle fibers, supporting its role as a contractile protein associated with myofibrillar structure.

MYL1 Antibody Heart IHC. Immunohistochemistry analysis of FFPE human heart tissue stained with MYL1 antibody detecting Myosin light chain 1. Cardiac muscle fibers show weak to minimal cytoplasmic staining compared to skeletal muscle, consistent with the preferential expression of MYL1 in fast skeletal muscle fibers rather than cardiac muscle. The staining highlights elongated muscle fibers with low-level signal, while surrounding stromal elements remain largely negative. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 6, 10 mM citrate buffer for 10-20 min and allow to cool before testing.

MYL1 Antibody HeLa FACS. Flow cytometry analysis of fixed and permeabilized human HeLa cells stained with MYL1 antibody detecting Myosin light chain 1. The antibody signal (green) overlaps closely with the isotype control (blue), indicating minimal intracellular detection of MYL1 in HeLa cells. This profile is consistent with the restricted expression of MYL1 to fast skeletal muscle fibers and its low or absent expression in non-muscle epithelial cell lines.