- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Myelin Basic Protein Antibody / MBP [clone IIE-13] (RQ5003)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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RQ5003 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 449 |
![Myelin Basic Protein Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in rat brain tissue.](https://www.nsjbio.com/plp/thumb_image/img-RQ5003-1.jpg?v=15478097 "Myelin Basic Protein Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in rat brain tissue.")
![Myelin Basic Protein Antibody in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in mouse brain tissue.](https://www.nsjbio.com/plp/thumb_image/img-RQ5003-2.jpg?v=1678526514 "Myelin Basic Protein Antibody in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in mouse brain tissue.")
![Myelin Basic Protein Antibody in Pig Brain IHC. Immunohistochemistry staining of FFPE pig brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in pig brain tissue.](https://www.nsjbio.com/plp/thumb_image/img-RQ5003-3.jpg?v=1277354203 "Myelin Basic Protein Antibody in Pig Brain IHC. Immunohistochemistry staining of FFPE pig brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in pig brain tissue.")
![Myelin Basic Protein Antibody in Rat and Mouse Brain WB. Western blot testing of rat and mouse brain tissue lysates with Myelin Basic Protein Antibody / MBP [clone IIE-13]. Lane 1: rat brain; Lane 2: mouse brain. Samples were separated by 12% SDS-PAGE under reducing conditions and the antibody was used at 1:500 overnight at 4oC. A goat anti-rabbit IgG-HRP secondary antibody was used at 1:5000. Specific bands corresponding to MBP isoforms were detected at approximately 16-22 kDa in both samples. This Myelin Basic Protein Antibody demonstrates detection of MBP in rat and mouse brain tissue.](https://www.nsjbio.com/plp/thumb_image/img-RQ5003-4.jpg?v=1531315377 "Myelin Basic Protein Antibody in Rat and Mouse Brain WB. Western blot testing of rat and mouse brain tissue lysates with Myelin Basic Protein Antibody / MBP [clone IIE-13]. Lane 1: rat brain; Lane 2: mouse brain. Samples were separated by 12% SDS-PAGE under reducing conditions and the antibody was used at 1:500 overnight at 4oC. A goat anti-rabbit IgG-HRP secondary antibody was used at 1:5000. Specific bands corresponding to MBP isoforms were detected at approximately 16-22 kDa in both samples. This Myelin Basic Protein Antibody demonstrates detection of MBP in rat and mouse brain tissue.")
| Species Reactivity | Human, Mouse, Rat, Pig |
| Format | Purified |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | IIE-13 |
| Purity | Affinity purified |
| UniProt | P02686 |
| Applications | Western Blot : 1:500-1:2000 Immunohistochemistry (FFPE) : 1:50-1:200 |
| Limitations | This Myelin Basic Protein antibody is available for research use only. |
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The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. [UniProt]
For broader research on myelin structure and MBP expression, see our Myelin Basic Protein Antibody / CNS Myelin Marker page.
Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
A synthetic peptide specific to human Myelin Basic Protein / MBP was used as the immunogen for the Myelin Basic Protein antibody.
Store the Myelin Basic Protein antibody at -20oC.
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![Myelin Basic Protein Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in rat brain tissue.](https://www.nsjbio.com/plp/all_image/img-RQ5003-1.jpg?v=910459341 "Click to enlarge")
![Myelin Basic Protein Antibody in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in mouse brain tissue.](https://www.nsjbio.com/plp/all_image/img-RQ5003-2.jpg?v=2044963140 id= "Click to enlarge")
![Myelin Basic Protein Antibody in Pig Brain IHC. Immunohistochemistry staining of FFPE pig brain tissue with Myelin Basic Protein Antibody / MBP [clone IIE-13]. HIER was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:50 overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody and DAB detection. This Myelin Basic Protein Antibody demonstrates MBP localization in pig brain tissue.](https://www.nsjbio.com/plp/all_image/img-RQ5003-3.jpg?v=1065766606 id= "Click to enlarge")
![Myelin Basic Protein Antibody in Rat and Mouse Brain WB. Western blot testing of rat and mouse brain tissue lysates with Myelin Basic Protein Antibody / MBP [clone IIE-13]. Lane 1: rat brain; Lane 2: mouse brain. Samples were separated by 12% SDS-PAGE under reducing conditions and the antibody was used at 1:500 overnight at 4oC. A goat anti-rabbit IgG-HRP secondary antibody was used at 1:5000. Specific bands corresponding to MBP isoforms were detected at approximately 16-22 kDa in both samples. This Myelin Basic Protein Antibody demonstrates detection of MBP in rat and mouse brain tissue.](https://www.nsjbio.com/plp/all_image/img-RQ5003-4.jpg?v=1592851564 id= "Click to enlarge")
