MTAP Antibody / Microarray Specificity Validated Antibody [clone MTAP/1813] (V3868)

MTAP Antibody A431 WB. Western blot analysis of human A431 cell lysate using MTAP Antibody (clone MTAP/1813) detects a band at approximately 26-38 kDa, consistent with the predicted molecular weight of Methylthioadenosine phosphorylase (MTAP) and reflecting the presence of multiple isoforms of this metabolic enzyme.

MTAP Antibody Human Kidney IHC. Immunohistochemistry analysis of FFPE human kidney tissue using MTAP Antibody (clone MTAP/1813) demonstrates HRP-DAB brown cytoplasmic staining in tubular epithelial cells, consistent with Methylthioadenosine phosphorylase (MTAP) expression as a metabolic enzyme involved in intracellular nucleotide and methionine salvage pathways, while glomerular structures show lower signal; nuclei are counterstained blue. HIER: steam sections in pH 6 citrate buffer for 20 min and allow to cool prior to staining.

MTAP Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MTAP Antibody (clone MTAP/1813) demonstrates highly specific detection of Methylthioadenosine phosphorylase (MTAP), a metabolic enzyme involved in the methionine salvage pathway. The antibody shows a dominant signal for MTAP with clear separation from all other proteins on the array, supporting strong target specificity of clone MTAP/1813. Z- and S-score: The Z-score represents the strength of signal generated when the antibody binds to a protein on the array, expressed as standard deviations above the mean signal, while the S-score reflects the difference between sequential Z-scores and indicates relative specificity compared to potential off-target interactions.

SDS-PAGE analysis of purified, BSA-free MTAP antibody (clone MTAP/1813) as confirmation of integrity and purity.

MTAP Antibody Multi-Cell Line WB. Western blot analysis of human HeLa, A431, HepG2, and HAP-1 cell lysates using MTAP Antibody (clone MTAP/1813) detects a band at approximately 26-38 kDa, consistent with the predicted molecular weight of Methylthioadenosine phosphorylase (MTAP) and reflecting the presence of multiple isoforms of this metabolic enzyme across diverse human cell lines.