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Home >> Antibodies >> MMP2 Antibody

MMP2 Antibody [clone 3A8-C1-E8] (F54029)

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  Catalog No Formulation Size Price (USD)  
Image F54029-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with MMP2 antibody at 1:50. DAPI was used to stain nuclei (blue).
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ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with MMP2 antibody at 1:50. DAPI was used to stain nuclei (blue).
Western blot testing of A) transfected (human MMP2, partial) and B) untransfected CHO-K1 cell lysate with MMP2 antibody at 1:2000. Expected molecular weight of the partial protein: ~30 kDa.
Western blot testing of mouse Raw264.7 cell lysate with MMP2 antibody at 1:200. Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 3A8-C1-E8
Purity Protein G affinity
UniProt P08253
Applications Western blot : 1:1000
ICC/IF : 1:50
Limitations This MMP2 antibody is available for research use only.
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Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant protein was used as the immunogen for this MMP2 antibody.

Storage

Store the MMP2 antibody at -20oC.

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