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Home >> Antibodies >> Mesothelin Antibody

Mesothelin Antibody [clone SPM143] (V7654)

  Catalog No Formulation Size Price (USD)  
Image V7654-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7654-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7654SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human endometrial carcinoma with Mesothelin antibody (clone SPM143). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung mesothelioma with Mesothelin antibody (clone SPM143). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name SPM143
Purity Protein G affinity chromatography
UniProt Q13421
Localization Cytoplasmic, cell surface, secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Mesothelin antibody is available for research use only.
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Description

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Application Notes

Optimal dilution of the Mesothelin antibody should be determined by the researcher.

Immunogen

A portion of amino acids 273-407 from the human protein was used as the immunogen for this MSLN antibody.

Storage

Store the Mesothelin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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