Mesothelin Antibody / Biotinylated Detection Antibody [clone MSLN/2131] (V3820BTN)

Biotinylated Mesothelin Antibody Endometrial Carcinoma IHC. Immunohistochemistry analysis of mesothelin expression in FFPE human endometrial carcinoma using biotinylated clone MSLN/2131. Tumor epithelial cells demonstrate membranous HRP-DAB brown staining with focal cytoplasmic signal, consistent with mesothelin surface localization. The biotinylated detection format enables enhanced signal intensity and improved visualization of tumor cell populations, particularly in regions with lower expression, while surrounding stromal elements remain low in background staining. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

Biotinylated Mesothelin Antibody Human Lung IHC. Immunohistochemistry analysis of mesothelin expression in FFPE human lung using biotinylated clone MSLN/2131. Focal membranous HRP-DAB brown staining is observed in epithelial structures with low background in surrounding tissue, consistent with limited physiologic mesothelin expression. The biotinylated detection format enhances signal visibility and improves contrast, supporting detection of low-level expression in normal tissue compartments. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

SDS-PAGE analysis of purified, BSA-free Mesothelin antibody (clone MSLN/2131) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Mesothelin antibody (clone MSLN/2131). These results demonstrate the foremost specificity of the MSLN/2131 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.