MCT5 Antibody / Monocarboxylate Transporter 5 Antibody (R31311)

MCT5 Antibody Human Kidney IHC. Immunohistochemistry analysis of FFPE human kidney tissue using MCT5 Antibody. Heat induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL). HRP detection with DAB chromogen was used for visualization. Strong membranous and cytoplasmic staining is observed throughout renal tubular epithelial cells, consistent with the expected expression pattern of MCT5 (SLC16A4), a proton-coupled monocarboxylate transporter involved in epithelial metabolite transport. These results support the use of MCT5 Antibody / Monocarboxylate Transporter 5 Antibody for studying MCT5 expression, SLC16A4 biology and monocarboxylate transport.

MCT5 Antibody Human Lung Cell IF. Immunofluorescence analysis of A549 cells using MCT5 Antibody (5 ug/mL). Cells were subjected to enzyme-mediated antigen retrieval, incubated overnight with primary antibody, and detected with a Fluoro488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue). MCT5 staining is distributed throughout the cytoplasm with enrichment along the plasma membrane, consistent with the expected localization of the SLC16A4 monocarboxylate transporter. These results support the use of MCT5 Antibody / Monocarboxylate Transporter 5 Antibody for studying MCT5 expression, SLC16A4 biology and epithelial monocarboxylate transport.

MCT5 Antibody Human, Rat and Mouse Kidney WB. Western blot analysis using MCT5 Antibody. Lane 1: human A549 cells; Lane 2: rat kidney tissue lysate; Lane 3: rat NRK cells; Lane 4: mouse kidney tissue lysate. A specific band is detected at approximately 54 kDa in all samples, consistent with the expected molecular weight of MCT5 (SLC16A4). Detection in both rodent kidney tissues and cultured epithelial cell lines supports the conserved expression of this proton-coupled monocarboxylate transporter across species. These results support the use of MCT5 Antibody / Monocarboxylate Transporter 5 Antibody for studying MCT5 expression, SLC16A4 biology and monocarboxylate transport.